医学分子生物学杂志2025，Vol.22Issue(3)：223-228,6.DOI:10.3870/j.issn.1672-8009.2025.03.003
circITCH靶向miR-106b对结直肠癌细胞增殖及侵袭的调控
CircITCH Regulates Proliferation and Invasion of Colorectal Cancer Cells via Targeting miR-106b
摘要
Abstract
Objective To study the effect of circITCH on the proliferation,invasion of colorec-tal cancer cells via targeting miR-106b.Methods Colorectal cancer cell lines SW620,HT-29,HCT116,Caco-2,SW480,and normal colon epithelial cell line NCM460 were cultured.The ex-pression levels of circITCH and miR-106b were detected,and HT-29 cells were selected for further experiments and were divided into 7 groups:Negative control(NC)group,circITCH group,miR-NC group,miR-106b group,NC plasmid+miR-NC group,circITCH+miR-NC group,and circITCH+miR-106b group.The A450 value,number of invasion cells and the expression levels of PTEN and B cell translocation gene 3(BTG3)were detected.Results The expression level of cir-cITCH in the SW620,HT-29,HCT116,Caco-2 and SW480 cells was lower than that in the NCM460 cell,while the expression level of miR-106b was higher in the NCM460 cell(P＜0.05).Dual-luciferase reporter gene assay showed that miR-106b could bind with circITCH reporter gene).The expression levels of circITCH,PTEN,BTG3 in the circITCH group were higher than those in the NC group,the expression level of miR-106b,A450 value,and the number of invasion cells were lower than those in the NC group(P＜0.05).The expression level of miR-106b,A450 value,and the number of invasion cells in the circITCH+miR-106b group were higher than those in the cir-cITCH+miR-NC group,the expression levels of PTEN,BTG3 were lower than those in the cir-cITCH+miR-NC group(P＜0.05).Conclusion CircITCH can inhibit the proliferation and inva-sion of colorectal cancer cells via targeting miR-106b.关键词
结直肠癌/环状RNA circITCH/miR-106b/增殖/侵袭Key words
colorectal cancer/circITCH/miR-106b/proliferation/invasion分类
临床医学引用本文复制引用
蒋林燕,沈晓雯,许美蓉..circITCH靶向miR-106b对结直肠癌细胞增殖及侵袭的调控[J].医学分子生物学杂志,2025,22(3):223-228,6.基金项目
南通市卫计委科研青年课题(No.QNZ2023060) This work was supported by a grant from the Nantong City Health and Family Planning Commission Research Youth Project(No.QNZ2023060) （No.QNZ2023060）
