医学分子生物学杂志2025，Vol.22Issue(3)：237-244,8.DOI:10.3870/j.issn.1672-8009.2025.03.005
维生素D3介导TIPE2调控慢性鼻-鼻窦炎伴鼻息肉中巨噬细胞的功能及表型
Vitamin D3 Mediates TIPE2 in Regulation of Macrophage Function and Phenotype in Chronic Rhinosinusitis with Nasal Polyps
摘要
Abstract
Objective To investigate the regulatory role of vitamin D3(VD3)-mediated tumor necrosis factor-alpha induced protein 8-like protein 2(TIPE2)on macrophage function and phenotype in chronic rhinosinusitis with nasal polyps(CRSwNP).Methods Peripheral blood sam-ples were collected from patients with CRSwNP as the CRSwNP group,and from patients under went surgery for isolated nasal septal deviation as the Control group.RT-qPCR and Western blotting were used to detect the expression levels of TIPE2 and Arginase-1(Arg-1)in peripheral blood,and chemiluminescence was used to detect the changes in VD3 concentration in peripheral blood.J774A.1 macrophages were cultured in vitro and divided into 5 groups:silenced TIPE2(si-TIPE2#2)group and its negative control(si-NC)group,negative control for TIPE2 overexpres-sion(oe-NC)group,oe-NC combined with VD3 treatment group(oe-NC+VD3 group),and overexpressed TIPE2 recombinant vector(oe-TIPE2)combined with VD3 treatment group(oe-TIPE2+VD3 group).In addition,a mouse model of CRSwNP was established,with CRSwNP mice di-vided into 3 groups:empty vector treatment group(CRSwNP+empty vector group),CRSwNP+oe-TIPE2 group,and CRSwNP+oe-TIPE2+VD3 group.Peripheral blood and macrophages were collected from each group of mice.RT-qPCR and Western blotting were used to detect the expression levels of TIPE2 and Arg-1 in macrophages.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin(IL)-4 and IL-13 in cell culture supernatants and mouse sera in each group.Results Compared to those in the control group,VD3 concentration was decreased in the CRSw-NP group,while the expression levels of TIPE2 and Arg-1,as well as the serum concentrations of IL-4 and IL-13,were upregulated(all P＜0.05).There was a significant positive correlation between Arg-1 and TIPE2 levels(r=0.994,P＜0.01),and both Arg-1 and TIPE2 levels were significantly negatively correlated with VD3 concentration(r=-0.652,P＜0.01;r=-0.745,P＜0.01).Compared to those in the si-NC group,the expression levels of Arg-1 and IL-4 and IL-13 concentrations decreased in the si-TIPE2#2 group(all P＜0.05).Compared to those in the oe-NC group,the expression levels of TIPE2 and Arg-1,as well as IL-4 and IL-13 concentrations,decreased in the oe-NC+VD3 group(all P＜0.05).Moreover,compared to those in the oe-NC+VD3 group,the expression levels of TIPE2 and Arg-1,as well as IL-4 and IL-13 concentrations,increased in the oe-TIPE2+VD3 group(all P＜0.05).Compared to those in the CRSwNP+emptyvector group,the expression levels of TIPE2 and Arg-1 in-creased in the CRSwNP+oe-TIPE2 group,while VD3 concentration decreased and the serum concentra-tions of IL-4 and IL-13 increased(all P＜0.05).Compared to those in the CRSwNP+oe-TIPE2 group,the expression levels of TIPE2 and Arg-1 decreased in the CRSwNP+oe-TIPE2+VD3 group,while the VD3 concentration increased and the serum concentrations of IL-4 and IL-13 decreased(all P＜0.05).Conclusion VD3 inhibits the expression of Arg-1,IL-4,and IL-13 in CRSwNP by downregulating TIPE2 expression.关键词
慢性鼻-鼻窦炎伴鼻息肉/维生素D3/巨噬细胞极化/肿瘤坏死因子α诱导蛋白8样蛋白2/炎症反应Key words
chronic rhinosinusitis with nasal polyps/vitamin D3/macrophage polarization/tumor necrosis factor-alpha induced protein 8-like protein 2/inflammatory response分类
药学引用本文复制引用
黄小荣,谭晖,陈娟,卡迪丽娅·木拉提,张瑾,陈伦牮..维生素D3介导TIPE2调控慢性鼻-鼻窦炎伴鼻息肉中巨噬细胞的功能及表型[J].医学分子生物学杂志,2025,22(3):237-244,8.基金项目
新疆维吾尔自治区自然科学基金(No.2022D01C588) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2022D01C588) （No.2022D01C588）
