医学分子生物学杂志2025，Vol.22Issue(3)：245-251,7.DOI:10.3870/j.issn.1672-8009.2025.03.006
脂肪干细胞来源外泌体通过miR-654-3p促进牙周韧带干细胞成骨分化
Adipose Stem Cell-derived Exosomes Promote Osteogenic Differentia-tion of Periodontal Ligament Stem Cells through miR-654-3p
摘要
Abstract
Objective To investigate the effect of adipose stem cells-derived exosomes(AD-SCs-Exo)on osteogenic differentiation of periodontal ligament stem cells(PDLSCs)through the miR-654-3p pathway.Methods Human ADSCs were isolated,identified,and cultured in vitro,and exosomes were isolated.Human PDLSCs were isolated in vitro,and the miR-654-3p inhibitor or its negative control(inhibitor NC)were transferred into cells,and cultured with or without a com-bined osteogenic induction solution.Cells were divided into 6 groups:Blank group,ADSCs-Exo group,inhibitor NC group,inhibitor NC+ADSCs-Exo group,miR-654-3p inhibitor group,miR-654-3p inhibitor+ADSCs-Exo group.After 7 days of induction,the expression levels of miR-654-3p,osteocalcin(OCN)and type Ⅰ collagen(COL1A1)mRNA in cells were detected by RT-qPCR,and ALP activity was detected in cells by alkaline phosphatase(ALP)activity kit.After 21 days of induction,the alizarin red staining method was used to detect the calcium nodules'forma-tion ability of cells,and the expression levels of OCN and COL1A1 protein were detected by West-ern blotting.Results Compared with those in the Blank group or inhibitor NC group,the expres-sion level of miR-654-3p,the activity of ALP,the expression levels of OCN and COL1A1 mRNA and protein in the ADSCs-Exo group were increased(P＜0.05),and the formation ability of calci-um nodules was enhanced(P＜0.05),and the expression level of miR-654-3p was decreased in the miR-654-3p inhibitor group.Compared with those in the inhibitor NC+ADSCs-Exo group,the ALP activity,the expression levels of OCN and COL1A1 mRNA and protein in the miR-654-3p in-hibitor+ADSCs-Exo group were decreased(P＜0.05),and the formation ability of calcium nod-ules was decreased(P＜0.05).Conclusion ADSCs-Exo promote osteogenic differentiation of PDLSCs by up-regulating the expression of miR-654-3p.关键词
脂肪干细胞/外泌体/牙周韧带干细胞/miR-654-3p/成骨分化Key words
adipose stem cells/exosomes/periodontal ligament stem cells/miR-654-3p/osteogenic differentiation分类
口腔医学引用本文复制引用
杨伊汨,王巍,陈洁点,安冰韬,温颖..脂肪干细胞来源外泌体通过miR-654-3p促进牙周韧带干细胞成骨分化[J].医学分子生物学杂志,2025,22(3):245-251,7.基金项目
北京市科技计划课题(No.Z220102302312310) This work was supported by a grant from the Beijing Science and Technology Project(No.Z220102302312310) （No.Z220102302312310）
