医学分子生物学杂志2025，Vol.22Issue(3)：252-258,7.DOI:10.3870/j.issn.1672-8009.2025.03.007
乌头汤调节Ahr/LOC101928120/SHC1信号通路抑制炎症和氧化应激对人尿源性干细胞的损伤
Wutou Decoction Inhibits Inflammation and Oxidative Stress Induced Injury of Human Urogenic Stem Cells by Ahr/LOC101928120/SHC1 Signaling Pathway
摘要
Abstract
Objective To investigate the mechanism by which Wutou decoction(WTD)in-hibits inflammatory and oxidative stress induced injury of human urogenic stem cells(UdSC)by modulating the aryl hydrocarbon receptor(Ahr)/long non-coding RNA(LncRNA)LOC101928120/SHC articulin 1(SHC1)signaling pathway.Methods UdSC cells were divided into 5 groups Control group,IL-1β group,IL-1β+WTD group,H2O2 group,and H2O2+WTD group.CellTiterGlo assay and flow cytometry were applied to detect the proliferation and apoptosis in the cells of each group.qRT-PCR was applied to detect the expression levels of Ahr,LOC101928120,and SHC1 mRNA.Western blotting was applied to detect the expression levels of Ahr,HDAC1,SHC1,COL2,and SOX-9.ELISA was applied to detect the levels of IL-6,TNF-α,and MMP-13 in inflammation-induced UdSC cells.DCFH-DA fluorescent probe was applied to detect the ROS level in oxidative stress-induced UdSC cells.Results The A560 nmvalue,the expres-sion levels of Ahr mRNA and protein,LOC101928120,COL2,SOX-9 protein in the IL-1 β group were lower than those in the Control group,and the values of above indexes in the IL-1 β+WTD group were higher than those in the IL-1 β group(P＜0.05).The apoptosis rate,the levels of IL-6,TNF-α,MMP-13,and the expression levels of SHC1 mRNA and protein and HDAC1 protein in the IL-1 β group were higher than those in the control group,and the values of above indexes in the IL-1β+WTD group were lower than those in the IL-1 β group(P＜0.05).TheA560nm value,the ex-pression levels of Ahr mRNA and protein,LOC101928120,COL2 and SOX-9 proteins in the H2O2 group were lower than those in the Control group,and the values of above indexes in the H2O2+WTD group were higher than those in the H2O2 group(P＜0.05).The apoptosis rate,the propor-tion of ROS-positive cells,and the expression levels of SHC1 mRNA and protein and HDAC1 pro-tein were higher than those in the Control group,and the above values in the H2O2+WTD group were lower than those in H2O2 group(P＜0.05).Conclusion WTD inhibits inflammatory and oxi-dative stress-induced injury in human UdSC,the mechanism may be achieved by modulating the Ahr/LOC101928120/SHC1 signaling pathway.关键词
乌头汤/Ahr/LOC101928120/SHC1信号通路/炎症/氧化应激/人尿源性干细胞Key words
Wutou decoction/Ahr/LOC 101928120/SHC1 signaling pathway/inflammation/oxidative stress/human urogenic stem cells分类
临床医学引用本文复制引用
吴丹,童亮明,李茜,周平,李洁芳..乌头汤调节Ahr/LOC101928120/SHC1信号通路抑制炎症和氧化应激对人尿源性干细胞的损伤[J].医学分子生物学杂志,2025,22(3):252-258,7.基金项目
湖南省自然科学基金(No.2023JJ60384) This work was supported by a grand from the Natural Science Foundation of Hunan Province(No.2023JJ60384) （No.2023JJ60384）
