医学分子生物学杂志2025，Vol.22Issue(3)：271-276,6.DOI:10.3870/j.issn.1672-8009.2025.03.010
SIRT3在儿童急性髓系白血病中的作用
Role of SIRT3 in Children with Acute Myeloid Leukemia
摘要
Abstract
Objective To explore the effect of silent information regulator 3(SIRT3)in chil-dren with acute myeloid leukemia(AML).Methods The expression level of SIRT3 in bone mar-row cells of AML children and peripheral blood leukocytes of healthy blood donor children was de-tected.SIRT3 expression was knocked down in AML cells.The cells' proliferation,apoptosis,oxi-dative stress indexes,and protein expression levels of the Wnt/β-catenin signaling pathway were detected.Results Compared with those in the normal children group,the expression levels of SIRT3 mRNA and protein were increased in the AML children group(P＜0.05).After knocking down SIRT3 expression in AML cells,the cells proliferation,the colony formation ability,the levels of glutathione(GSH)and superoxide dismutase(SOD),the total antioxidant capacity(T-AOC),the expression level of β-catenin,and the transcription activity of T cell factor/lymphocyte enhancer factor(TCF/LEF)were decreased,while the apoptosis rate,the levels of reactive oxygen species(ROS),malond-ialdehyde(MDA)and phosphorylated glycogen synthase kinase 3β(p-GSK-3β)were increased(P＜0.05),the GSK-3β inhibitor TWS119 could partially reverse the above effects.Conclusion Knoc-king down SIRT3 can inhibit the growth of AML cells and increase oxidative stress level,which may be related to the inhibition of Wnt/β-catenin signaling pathways.关键词
急性髓系白血病/沉默信息调节因子3/细胞凋亡/氧化应激/Wnt/β-catenin信号通路Key words
acute myeloid leukemia/silent information regulator 3/apoptosis/oxidative stress/Wnt/β-catenin signaling pathway分类
临床医学引用本文复制引用
任华,郝强,郭丽,郑素华,卫雷..SIRT3在儿童急性髓系白血病中的作用[J].医学分子生物学杂志,2025,22(3):271-276,6.基金项目
河北省医学科学研究课题(No.20220520) This work was supported by a grant from the Medical Science Research Project of Hebei Province(No.0220520) （No.20220520）
