广西植物2025，Vol.45Issue(5)：886-902,17.DOI:10.11931/guihaia.gxzw202409031
鸢尾属31个分类群的花粉形态及其分类学意义
Pollen morphology of 31 taxa of genus Iris(Iridaceae)and its taxonomic implications
摘要
Abstract
To investigate the pollen morphology and its potential taxonomic implications for genus Iris L.(Iridaceae),the pollen grains from 31 taxa across three subgenera of Iris were used as materials.The materials were prepared using critical point drying method,followed by scanning electron microscopy(SEM)observation and systematic cluster analysis of pollen morphology.The results were as follows:(1)All the pollen grains of the 31 taxa were monad and heteropolar,oblate,suboblate or spheroidal in shape,with medium or large size.The sulcus membrane was smooth or ornamented.The pollen grains were semitectate or atectate,with different types of heterobrochate(rarely gemmate-clavate)exine ornamentation.(2)The pollen grains of Iris tigridia were inaperturate,while those of the remaining taxa were sulcate.Zonasulcate pollen grains were found in I.bungei,I.kobayashii and I.ventricosa.For the first time the disulcate pollen grains were found in I.hookeri in genus Iris.(3)Cluster analysis showed that at a squared Euclidean distance of 10,31 taxa could be clustered into seven types,i.e.,Sibirica type,Germanica type,Pumila type,Ruthenica type,Laevigata type,Spuria type and Tigridia type.The taxa studied cluster basically in accordance with the subgenera,sections and series of the morphotaxonomy.Of the seven types,the Germanica type had the largest pollen grains and a heterobrochate reticulate exine with the largest lumina,while the Ruthenica type had the smallest pollen grains and a microreticulate or nanoreticulate exine.The Pumila type was characterized by atectate pollen grains with a gemmate-clavate exine ornamentation and the Spuria type was characterized by suboblate shape pollen grains with an ornamented sulcus membrane.(4)The possible evolutionary trends of the pollen morphology of three subgenera were proposed:the subgenus Limniris was more primitive than the subgenus Iris,series Ruthenicae was the most primitive taxon among the species studied,and subgenus Pardanthopsis and section Lophiris could be the intermediate types in the transition of the subgenus Limniris to the subgenus Iris.(5)The critical-point drying method and 2,2-dimethoxypropane(DMP)direct method were suitable methods for the preparation of pollen micromorphology of the genus Iris.The results of this study indicate that the pollen morphology of 31 Iris taxa reflects both consistency and differences.The morphology data of pollen can be used as supplementary characteristics to identify the subgenera,sections and series,providing reliable palynological evidence.关键词
鸢尾属/临界点干燥法/花粉形态/分类学意义/扫描电镜Key words
Iris/critical-point drying method/pollen morphology/taxonomic implications/SEM分类
农业科技引用本文复制引用
朱莹,宋华,李凯,张蕾,王白冰..鸢尾属31个分类群的花粉形态及其分类学意义[J].广西植物,2025,45(5):886-902,17.基金项目
北京市公园管理中心科技项目(ZX2019010,ZX2023011). （ZX2019010,ZX2023011）
