广西植物2025，Vol.45Issue(5)：903-915,13.DOI:10.11931/guihaia.gxzw202405046
菠萝蜜WRKY基因家族注释及在低温胁迫下的表达模式分析
Identification and expression pattern analysis of WRKY gene family in jackfruit under low temperature stress
摘要
Abstract
The WRKY transcription factors family plays an important role in plant biological and abiotic stress responses,a total of 61 WRKY gene family members of jackfruit(Artocarpus heterophyllus)were identified based on the whole genome of jackfruit,and the expression profile of WRKY gene family members in jackfruit under low temperature stress were analyzed by bioinformatics method and quantitative real-time fluorescence PCR(qRT-PCR).The results were as follows:(1)Phylogenetic tree analysis showed that WRKY gene family members of jackfruit,Arabidopsis thaliana and rice(Oryza sativa)were divided into four subfamilies.(2)Chromosome localization results showed that 61 AhWRKY gene family members were unevenly distributed on 23 chromosomes.(3)Analyses of conserved motifs and gene structure showed that AhWRKY gene family members located in the same subfamily had similar conserved motifs and gene structure.(4)Phylogenetic analysis revealed 124 pairs of fragment duplicated genes within AhWRKY gene family members;inter-species analysis showed that jackfruit exhibited a greater number of homologous gene pairs with Arabidopsis thaliana than rice and fig(Ficus carica);while there were fewer homologous gene pairs between rice and fig,with only eight genes forming homologous gene pairs among the three species.(5)Transcriptomic analysis of different varieties of jackfruit under low temperature stress showed that the expression patterns of WRKY gene family members differed in different varieties.Moreover,qRT-PCR analysis further verified this result,indicating that WRKY gene family members played an important role in response to low temperature stress in jackfruit of different varieties.This study provides new insights into the evolution and function of WRKY gene family members,and lays a foundation for functional research and utilization of WRKY gene family members in jackfruit.关键词
菠萝蜜/WRKY基因家族/低温胁迫/表达模式/生物信息学分析Key words
jackfruit/WRKY gene family/low temperature stress/expression pattern/bioinformatics analysis分类
生物学引用本文复制引用
马湘玮,庞新华,朱鹏锦,杜英俊,宋奇琦,叶维雁,唐秀观,何江,钟云婕,欧景莉..菠萝蜜WRKY基因家族注释及在低温胁迫下的表达模式分析[J].广西植物,2025,45(5):903-915,13.基金项目
广西重点研发计划项目(桂科AB21220003) （桂科AB21220003）
广西农科院基本科研业务专项(桂农科2023YM15) （桂农科2023YM15）
广西农业科技创新联盟项目(桂农科盟202404). （桂农科盟202404）
