广西植物2025，Vol.45Issue(5)：916-930,15.DOI:10.11931/guihaia.gxzw202402013
垂穗披碱草TCP转录因子家族鉴定及激素响应模式分析
Identification of TCP transcription factor family and analysis of phytohormone response patterns in Elymus nutans
摘要
Abstract
Elymus nutans,a high-quality forage distributed on the Qinghai-Tibetan Plateau,holds high ecological and economic values.TCP transcription factors,a plant-specific transcription factor family,play a crucial regulatory role in plant growth and development processes,including leaf development,lateral branch morphogenesis,and phytohormone synthesis and signal transduction pathways.To identify the TCP transcription factor family members of E.nutans,single molecule real-time(SMRT)sequencing technology was used to obtain full-length transcriptome data from E.nutans.Meanwhile,the Illumina sequencing platform was employed to investigate the response patterns of E.nutans under four phytohormone treatments.The results were as follows:(1)A total of 90 956 non-redundant full-length non-chimeric(FLNC)transcripts were obtained.(2)A total of 26 EnTCPs were identified based on the full-length transcriptome data.Bioinformatics analysis revealed a diverse range of amino acids(186 to 575 aa),with all TCP proteins predicted to be located in the nucleus.(3)Based on the branching of the phylogenetic tree,the 26 EnTCPs were divided into Class Ⅰ subfamily,Class Ⅱ-a(CIN)subfamily,and Class Ⅱ-b(CYC/TB1)subfamily.Conserved motifs analysis revealed that all EnTCPs possessed a TCP domain.(4)Expression pattern analysis suggested that the expression of seven EnTCPs family members(En108950,En35573,En10347,En16325,En128790,En10346,and En14028)was up-regulated/down-regulated under different phytohormone treatments,suggesting their potential involvement in hormone synthesis pathways.qRT-PCR analysis results showed that En35573 and En14028 were involved in auxin response,while En108950,En10347,En128790,En10346,and En14028 were all involved in cytokinin response.En14028 was involved in abscisic acid response,En108950 was involved in jasmonic acid response,and En16325 might be involved in multiple hormone signaling pathways.These findings provide a foundation for further exploration of the function of the EnTCPs gene and serve as a reference for studying the molecular mechanisms of EnTCPs'response to phytohormones.关键词
垂穗披碱草/TCP转录因子家族/全长转录组/外源植物激素/表达模式Key words
Elymus nutans/TCP transcription factor family/full-length transcriptome/exogenous phytohormones/expression patterns分类
生物学引用本文复制引用
彭晓梅,孟晨,GARCÍA-CAPARRÓS Pedro,张宇,杨永平,孙旭东..垂穗披碱草TCP转录因子家族鉴定及激素响应模式分析[J].广西植物,2025,45(5):916-930,15.基金项目
国家自然科学基金(32370405). （32370405）
