广西植物2025，Vol.45Issue(5)：931-942,12.DOI:10.11931/guihaia.gxzw202405051
太子参R2R3-MYB转录因子鉴定及表达分析
Identification and expression analysis of R2R3-MYB transcription factor in Pseudostellaria heterophylla
摘要
Abstract
R2R3-MYB transcription factor plays an important role in plant growth and development,stress response,and secondary metabolism.In order to explore the temporal and spatial expression pattern of R2R3-MYB transcription factors in Pseudostellaria heterophylla and screen R2R3-MYB transcription factors involved in the regulation of heterophyllin B biosynthesis,15 R2R3-MYB transcription factors were identified from P.heterophylla based on the full-length transcriptome database.Their physicochemical properties,conserved motifs and phylogenetic relationships were analyzed by bioinformatics methods,and their tissue expression and abscisic acid induced expression patterns were studied by qRT-PCR.The results were as follows:(1)A total of 15 PhR2R3-MYB protein subcells were predicted to locate in cell nucleus,and most of them were hydrophilic unstable proteins.By constructing phylogenetic tree with Arabidopsis thaliana R2R3-MYB protein,PhR2R3-MYB proteins could be divided into eight subgroups,of which six subgroups can be clustered with A.thaliana R2R3-MYB transcription factors respectively.(2)PhR2R3-MYB gene had tissue expression specificity in Pseudostellaria heterophylla,and two of the genes,PhMYB4 and PhMYB8,were highly expressed in phloem of tuberous root.Correlation analysis showed that expression of PhMYB4 and PhMYB8 genes were significantly positively correlated with heterophyllin B content and prePhHB gene expression in P.heterophylla,suggesting that PhMYB4 and PhMYB8 might be involved in the regulation of heterophyllin B biosynthesis in P.heterophylla.(3)PhMYB4 and PhMYB8 genes were differentially expressed in response to ABA treatment for different time.The expression of PhMYB4 gene decreased first,then increased and then decreased,while the expression of PhMYB8 gene was inhibited with ABA treatment,indicating that both PhMYB4 and PhMYB8 genes respond to ABA signal.This study will lay a foundation for further investigation of the function of PhR2R3-MYB genes,and also provides a theoretical reference for revealing the mechanism of R2R3-MYB transcription factors involved in the regulation of heterophyllin B biosynthesis.关键词
太子参/R2R3-MYB转录因子/环肽B/生物信息学分析/表达分析Key words
Pseudostellaria heterophylla/R2R3-MYB transcription factor/heterophyllin B/bioinformatics analysis/expression analysis分类
生物学引用本文复制引用
黄以书,何华,周涛,潘琪,刘红霞,徐娇,欧小宏,江维克..太子参R2R3-MYB转录因子鉴定及表达分析[J].广西植物,2025,45(5):931-942,12.基金项目
国家自然科学基金地区项目(82160728,82160716) （82160728,82160716）
贵州省基础研究项目(黔科合基础-ZK[2022]一般464) （黔科合基础-ZK[2022]一般464）
贵州省教育厅高校科研平台项目(黔教技[2022]021号). （黔教技[2022]021号）
