河北医学2025，Vol.31Issue(5)：723-730,8.DOI:10.3969/j.issn.1006-6233.2025.05.04
LncRNA NORAD调控miR-136-5p/CBX4轴对急性髓系白血病细胞迁移和侵袭的影响
Impacts of LncRNA NORAD on Migration and Invasion of Acute Myeloid Leukemia Cells by Adjusting miR-136-5p/CBX4 Axis
摘要
Abstract
Objective:To investigate the impacts of long non-coding RNA(LncRNA)non-coding RNA activated by DNA damage(NORAD)on migration and invasion of acute myeloid leukemia(AML)cells by adjusting microRNA-136-5p(miR-136-5p)/chromobox protein homolog 4(CBX4)axis.Methods:Hu-man AML cell line KG1a was used as the research subject and divided into control group,sh-NC group,sh-NORAD group,sh-NORAD+NC-inhibitor group,and sh-NORAD+miR-136-5p-inhibitor group,with six replicates in each group.The expressions of LncRNA NORAD,miR-136-5p,and CBX4 mRNA in KG1a cell lysate were detected by qRT-PCR.Plate cloning,scratch assay,and Transwell assay were performed to detect the impacts of knocking down LncRNA NORAD on proliferation,migration,and invasion of KG1a cells.Moreover,Western blot was used to detect protein expression.Results:Compared with sh-NC group and control group,the clone number,scratch healing rate,invasion number,LncRNA NORAD,CBX4 mR-NA and protein,MMP-9 were decreased in sh-NORAD group,and the expression of miR-136-5p was in-creased(P<0.05).Compared with sh-NORAD group and sh-NORAD+NC-inhibitor group,the clone num-ber,scratch healing rate,invasion number,CBX4 mRNA and protein and MMP-9 of sh-NORAD+miR-136-5p-inhibitor group were increased.The expression of miR-136-5p was decreased(P<0.05).LncRNA NORAD could target the negative regulation of miR-136-5p,and miR-136-5p could target the negative reg-ulation of CBX4.Conclusion:LncRNA NORAD can regulate the miR-136-5p/CBX4 axis to promote migra-tion and invasion of AML cells.关键词
急性髓系白血病/长链非编码RNA/DNA损伤诱导的非编码RNA/微小RNA-136-5p/多梳蛋白4/迁移/侵袭Key words
Acute myeloid leukemia/Long non-coding RNA/Non-coding RNA activated by DNA damage/MicroRNA-136-5p/Chromobox protein homolog 4/Migration/Invasion引用本文复制引用
李彦卿,许晓炜,刘江,王莉丽,王磊..LncRNA NORAD调控miR-136-5p/CBX4轴对急性髓系白血病细胞迁移和侵袭的影响[J].河北医学,2025,31(5):723-730,8.基金项目
河北省医学科学研究课题计划项目,(编号:20221900) （编号:20221900）
张家口市科学技术研究与发展计划项目,(编号:1821112D) （编号:1821112D）
