河北医学2025，Vol.31Issue(5)：730-735,6.DOI:10.3969/j.issn.1006-6233.2025.05.05
丹参酮干预TLRs信号通路对重症脓毒血症心肺损伤保护作用的实验研究
Experimental Research on the Protective Effect of Tanshinone Intervention on TLRs Signaling Pathway in Severe Sepsis Induced Cardiorespiratory Injury
摘要
Abstract
Objective:To investigate the protective effects of tanshinone on heart and lung injury in-duced by severe sepsis and its association with the Toll-like receptor(TLRs)signaling pathway.Methods:A total of 80 male C57BL/6 mice were randomly divided into four groups:normal control group,sepsis model group,low-dose tanshinone(low-tan)group,and high-dose tanshinone(high-tan)group.Sepsis was in-duced in the model group by intraperitoneal injection of lipopolysaccharide(LPS).The low-tan and high-tan groups were treated with low and high doses of tanshinone,respectively,1 hour before model induction.After 24 hours of modeling,heart and lung function,oxidative stress markers,TLRs signaling pathway-related pro-tein expression,and pathological changes were assessed.Results:Compared with the control group,the air-way resistance in the model group was significantly increased(P<0.01),and the lung compliance was signifi-cantly reduced(P<0.01);compared with the model group,the airway resistance in the low-tan group and high-tan group was reduced(P<0.01),and the lung compliance was increased(P<0.01),and the im-provement in the high-tan group was more significant.The MDA level in heart and lung tissues of the model group was increased(P<0.01),and the SOD level was reduced(P<0.01);compared with the model group,the MDA level in the low-tan group and high-tan group was decreased(P<0.01),and the SOD level was increased(P<0.01),and the improvement in the high-tan group was more significant.The expression levels of TLR4,MyD88,and NF-κB proteins in heart and lung tissues of the model group were higher than those in the control group(P<0.001);the expression levels of these proteins in the low-tan group and high-tan group were lower than those in the model group(P<0.01 or P<0.001),and the reduction in the high-tan group was more obvious.In the control group,cardiomyocytes were arranged neatly and the structure was nor-mal without obvious pathological changes;in the model group,cardiomyocytes were edematous and degenera-ted,and there were inflammatory cell infiltrations in the interstitium,and myocardial fibrosis may occur;in the low-tan group,the degree of cardiomyocyte damage was reduced compared with the model group,and the infiltration of inflammatory cells was reduced;in the high-tan group,the morphology of cardiomyocytes was close to normal and the inflammatory reaction was mild.In the control group,the alveolar structure was com-plete and there was no obvious inflammatory cell infiltration;in the model group,the alveolar wall was thick-ened,inflammatory cell infiltration was obvious,and the alveolar structure was damaged;in the low-tan group,alveolar inflammation was reduced and structural damage was improved;in the high-tan group,the alveolar structure was basically restored to normal and there was very little inflammatory cell infiltration.Con-clusion:Danshentone significantly reduced cardiopulmonary injury caused by severe sepsis by inhibiting TLRs signaling pathway and reducing oxidative stress response.关键词
脓毒血症/丹参酮/TLRs信号通路/心肺损伤Key words
Sepsis/Tanshinone/TLRs signaling pathway/Cardiopulmonary injury引用本文复制引用
张洪胜,孔媛敏,纪艳飞,苏晓月,邢瑞伟..丹参酮干预TLRs信号通路对重症脓毒血症心肺损伤保护作用的实验研究[J].河北医学,2025,31(5):730-735,6.基金项目
河北省中医药类科学研究课题计划项目,(编号:2022246) （编号:2022246）
