中医学报2025，Vol.40Issue(6)：1318-1324,7.DOI:10.16368/j.issn.1674-8999.2025.06.212
肾气丸及其拆方对糖尿病肾脏病小鼠肾功能与AQP1表达的影响
Effects of Shenqi Pill and Its Modified Formula on Renal Function and AQP1 Expression in Mice with Diabetic Kidney Disease
摘要
Abstract
Objective:To investigate the effects of Shenqi Pill and its modified formula on renal function and aquaporins(AQPs)in dia-betic kidney disease(DKD)in mice.Methods:Twenty-four db/db mice were randomly divided into the model group(Mo group),Shenqi Pill group(S-R group,10.4 g·kg-1),Shenqi Pill-kidney tonic group(S-T group,8.1 g·kg-1),and Shenqi Pill-diu-retic group(S-D group,2.3 g·kg-1),with six mice in each group;and six db/m mice were taken as the control group(C group).Fasting blood glucose(FBG)and glycosylated serum protein(GSP)levels were measured by taking the tail tip blood of mice.Serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),and triglyceride(TG)were measured by ELISA.density lipoprotein cholesterol(HDL-C),serun creatinine(SCr),blood urea nitrogen(BUN),microalbumin(mAlB),urine creatinine(UCr),and urinary creatinine(UCr).The levels of serun creatinine(SCr),urea nitrogen(bloodurea nitrogen,BUN),microalbumin(mAlB),urinary creatinine(UCr),and 24 hour urinary protein(UP)were quantified at 24 h.The pathological morphology of renal cortical tissues was detected by HE staining method,and the protein ex-pression level of AQP1 in renal tissues was detected by Western Blot method.Results:Compared with group C,the FBG level of mice in Mo group was increased(P＜0.01);compared with Mo group,the FBG level of mice in S-D group was decreased(P＜0.01).Com-pared with group C,the level of GSP in mice in Mo group was increased(P＜0.01);compared with Mo group,the level of GSP in mice in S-R and S-D groups was decreased(P＜0.05).Compared with group C,the serum TC,TG,LDL-C levels of mice in Mo group were increased,and HDL-C levels were decreased(P＜0.05);compared with Mo group,the serum TC,TG,LDL-C levels of mice in S-R group and S-T group were decreased,and HDL-C levels were increased(P＜0.05).Compared with group C,mice in Mo group had increased levels of SCr,BUN,mAlB,and UP,and decreased levels of UCr(P＜0.01);mice in S-D group had decreased levels of SCr,BUN,mAlB,UCr,and UP compared with Mo group(P＜0.05);and mice in S-R and S-T groups had decreased levels of SCr,BUN,mAlB,and UP compared with Mo group(P＜0.05).HE staining showed that there were no obvious pathological changes in the renal cortex of mice in group C;glomerular thylakoid hyperplasia in the renal cortex of mice in group Mo,accompanied by matrix deposition;and glomerular thylakoid hyperplasia in the cortical area only was seen in mice in groups S-R,S-T,and S-D.Compared with group C,the renal AQP1 protein expression level of mice in Mo group was reduced(P＜0.01);compared with Mo group,the renal AQP1 protein expression level of mice in S-R group was increased(P＜0.05).Conclusion:Shenqi Pill and its modified formula can improve renal injury in DKD mice,and its mechanism may be related to the regulation of AQP1 protein expression.关键词
肾气丸/肾气丸拆方/糖尿病肾脏病/水通道蛋白/AQP1/肾功能Key words
Shenqi Pill/modified formula of Shenqi Pill/diabetic kidney disease/aquaporin/AQP1/renal function分类
医药卫生引用本文复制引用
裴海鹏,谢毅强,李凯,王献,张琳,邵恩茜,王楠,任天爱,冯鷺宝,梁一璐,吴培宇..肾气丸及其拆方对糖尿病肾脏病小鼠肾功能与AQP1表达的影响[J].中医学报,2025,40(6):1318-1324,7.基金项目
国家自然科学基金项目(82160897,82205087) （82160897,82205087）
海南省"南海新星"教育平台项目(JYNHXX2023-16G) （JYNHXX2023-16G）
海南医学院2022年大学生创新创业训练计划项目(202211810015) （202211810015）
