河南农业科学2025，Vol.54Issue(5)：59-67,9.DOI:10.15933/j.cnki.1004-3268.2025.05.007
高去除性耐镉真菌的筛选及其对小麦幼苗生长和镉积累的影响
Screening of Cd-tolerant Fungi with High Cd Removal Ability and Their Effects on Wheat Growth and Cd Accumulation
摘要
Abstract
This study screened native fungi with high cadmium tolerance and removal performance from soil and plants contaminated with heavy metals in wheat fields,and produced solid fermentation remediation agents.Then it conducted wheat safety testing in healthy soil and investigated the effects of remediation agents on wheat growth and cadmium absorption under in-situ heavy metal pollution conditions.The results showed that four functional fungi(M6,M10,M7,M3)with tolerance of up to 600 μmol/L Cd2+concentration and high cadmium removal rate in liquid culture medium containing 50 mg/L Cd2+were isolated and screened,with removal rates of 99.7%,79.3%,64.1%,and 62.8%,respectively.They were identified as Fusarium sp.,Trichoderma harzianum,Alternaria sp.,and Chaetomium sp..In wheat safety testing,only M6 was found to inhibit the emergence rate and biomass of wheat.Under in-situ heavy metal contaminated soil,the addition of M3,M7,and M10 significantly increased the fresh weight(22.0%—43.7%)and dry weight(15.7%—64.4%)of wheat,meanwhile significantly reducing the accumulation of Cd in the wheat root(37.2%—53.4%).Among them,M7 treatment had the best effect.In summary,Chaetomium sp.M7 could promote the growth of wheat seedlings under cadmium stress and reduce cadmium accumulation,which could be used for soil heavy metal pollution control.关键词
小麦/真菌/重金属/镉/微生物修复Key words
Wheat/Fungi/Heavy metal/Cadmium/Microbial remediation分类
农业科技引用本文复制引用
李世行,高巍,李洋洋,孙世龙,班佳浩,聂兆君,耿月华,汪敏,赵鹏..高去除性耐镉真菌的筛选及其对小麦幼苗生长和镉积累的影响[J].河南农业科学,2025,54(5):59-67,9.基金项目
农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室项目(2024-cdhjwrfk-09) （2024-cdhjwrfk-09）
河南省高等学校重点科研项目(22A210018) （22A210018）
河南省自然科学基金项目(242300420158) （242300420158）
