核农学报2025，Vol.39Issue(7)：1376-1383,8.DOI:10.11869/j.issn.1000-8551.2025.07.1376
核辐射诱变创制低镉水稻新种质
Creation of Novel Rice Germplasm with Low-Cadmium by Nuclear Radiation Mutagenesis
摘要
Abstract
Radiation mutagenesis is widely applied in generating valuable genetic resources for functional genomics and varietal development.To improve the efficiency of screening and identify desirable mutations from large mutagenized populations,we developed a multi-gene liquid phase probe capture technology combined with high-throughput sequencing method,according to the variation characteristics of nuclear radiation-induced mutations.With this method,genetic variations with target genes can be selected with seedlings grown in the laboratory under hydroponic conditions.Based on this technology,OsNramp5 mutant plants-a heavy metal cadmium transport transmembrane protein gene were successfully identified from rice mutagenic population.Furthermore,the obtained homozygous mutants were transplanted into pot cultures and paddy fields with moderate Cd contamination.The cadmium content in the brown rice of OsNramp5 mutants were below the national limit standard of 0.2 mg·kg-1,much lower than that of wild type.This study establishes a high-efficiency screening technology for targeting specific traits with radiation-induced mutagenesis,and significantly enhancing the accuracy for mutation identification.This approach provides a technical framework for high-throughput selection of desirable trait variations.关键词
辐射诱变/水稻/靶向筛选/高通量测序/种质资源Key words
radiation-induced mutagenesis/rice(Oryza sativa L.)/targeted screening/high-throughput sequencing/germplasm resources引用本文复制引用
张莉,张泓影,张逸妍,吕思维,张渊海,彭妙,陈哲红,杨震..核辐射诱变创制低镉水稻新种质[J].核农学报,2025,39(7):1376-1383,8.基金项目
湖南省科技创新计划资助(2023NK2008),科技揭榜挂帅湖南省重大科技攻关项目(2022NK1010),国家"十四五"重点研发专项项目(2022YFD1200702),核能开发科研项目"核辐射作物品种改良与害虫防控"(E11Z211GQ1) （2023NK2008）
