核农学报2025，Vol.39Issue(7)：1384-1391,中插1-中插3,11.DOI:10.11869/j.issn.1000-8551.2025.07.1384
快中子辐射下小麦突变群体的变异与鉴定
Variation and Identification in Fast Neutron-Irradiated Wheat Mutants
摘要
Abstract
To enhance the genetic diversity of wheat and develop novel germplasm resources,this study employed fast neutron radiation(1×1011 and 3×1011 n·cm-2)to treat Xinhua Mai 818 wheat seeds with two different doses.Among 9 848 M2 lines,2 562 exhibited significant phenotypic alterations in traits including leaves,stems,and spikes,resulting in a total mutation frequency of 26.02％.However,certain mutants,characterized by twisted,thin,and narrow leaf blades and double spike heads,were not stably inherited in subsequent generations,suggesting false positives.These mutants require multi-generation validation to confirm stability.In this study,mutant lines in M2 generation that did not exhibit significant variations in agronomic traits compared to control were furtherly analyzed on thousand kernel weight and total starch content,which are critical factors for yield traits.The findings revealed that 306 lines demonstrated significant variation in thousand kernel weight,among which 211 also exhibited significant variation in total starch content.Subsequently,the amylose content was quantified in the M3 generation,and seven mutant lines exhibiting a notably high-amylose content exceeding 30％were identified.In summary,this research successfully established a comprehensive library of fast neutron-induced wheat mutants with diverse traits and identified mutant lines with elevated amylose levels.This work lays a robust foundation for functional gene analysis,high-amylose germplasm resources development,and the selection and breeding of novel wheat varieties(lines).关键词
小麦/快中子辐射诱变/突变体/表型筛选/高直链淀粉Key words
wheat/fast neutrons radiation mutagenesis/mutants/phenotypic screening/high-amylose starch引用本文复制引用
苑沙沙,韩安琪,陈帅博,汪金玺,郭天财,李鸽子,王永华,康国章..快中子辐射下小麦突变群体的变异与鉴定[J].核农学报,2025,39(7):1384-1391,中插1-中插3,11.基金项目
国家重点研发计划(2021YFF1000203),国家自然科学基金(32171924) （2021YFF1000203）
