核农学报2025，Vol.39Issue(7)：1392-1405,14.DOI:10.11869/j.issn.1000-8551.2025.07.1392
广西百香果茎基腐病病原鉴定及抗性种质筛选
Identification of Fungal Pathogens Associated with Passion Fruit Stem Base Rot in Guangxi and Evaluation of Resistant Germplasm Sources
摘要
Abstract
To identify the key fungal pathogens associated with passion fruit stem base rot in Guangxi and to screen germplasm source resistant to passion fruit stem base rot,conventional pathogen isolation methods were employed to isolate and identify the pathogens responsible for passion fruit stem base rot collected from 6 cities in Guangxi,including Nanning,Yulin,and Guigang,and the pathogenicity of the isolates was assessed.The resistance to stem base rot were screened and ifentified in 34 passion fruit germplasm sources.A total of 23 pathogenic fungal isolates were characterized through ITS sequence analysis and confirmed through Koch's postulates.Morphological characteristics and the sequencing of the tef1-α and tub2 genes confirmed that Lasiodiplodia theobromae was predominant in all the studied regions of Guangxi with the exception of Yulin,indicating its widespread distribution throughout the province.Notably,strain BXG 2-2 exhibited the shortest incubation period of 4.00 days,with a 100％incidence rate while recording a disease index of 100,highlighting its aggressive virulence.Furthermore,an assessment conducted on 34 passion fruit germplasm sources revealed varyingresistance that 1 germplasm source was immune,3 were highly resistant,8 were moderately resistant,10 were susceptible,and 12 were highly susceptible.Notably,no significant difference in resistance was observed between the yellow fruit and purple fruit germplasm source.In conclusion,L.theobromae was the primary pathogen responsible for passion fruit stem base rot in Guangxi.The JTN,Hua J,Lvpi,and Qinzhouzhenmu No.1 have been identified as dominant resistant germplasm sources for managing this disease within passion fruit cultivation areas across Guangxi.The results of this study provide a theoretical foundation for scientific prevention and breeding passion fruit against stem base rot in Guangxi.关键词
百香果/茎基腐病/病原菌/抗性种质/广西Key words
passion fruit/stem base rot/fungal pathogen/resistant germplasm source/Guangxi引用本文复制引用
莫干辉,覃爱颖,陈善锋,韦广谭,谭奕为,苏文潘,张莉娟,张英俊..广西百香果茎基腐病病原鉴定及抗性种质筛选[J].核农学报,2025,39(7):1392-1405,14.基金项目
广西自然科学基金(2021GXNSFBA196005),广西科技重大专项(桂科AA22068091),广西农业科学院基本科研业务专项项目(桂农科2022JM94),南宁市科技重点研发计划(20232058) （2021GXNSFBA196005）
