核农学报2025，Vol.39Issue(7)：1425-1433,9.DOI:10.11869/j.issn.1000-8551.2025.07.1425
一株具解磷功能细菌菌株的鉴定及其生物学功能
Identification of a Bacterial Strain with Phosphorus-Solubilizing Function and Its Biological Function
摘要
Abstract
To screen candidate strains for the development of functional microbial inoculants,this study used the plate culture method and Mo sb anti-colorimetry method to isolate phosphate-solubilizing bacteria from 150 phyllospheric bacterial isolates.Morphological characteristics,physiological and biochemical properties,as well as 16S rDNA and gyrB gene sequence analysis were used to ascertain their taxonomic status.Furthermore,nitrogen fixation capability,antibacterial activity,and siderophile production were assessed.The results showed that 45 strains were selected from the 150 Phyllotropic bacteria.Among these,strain G13-2 exhibited the strongest phosphorus-solubilizing capacity,demonstrating a transparent circle radius of 7.63 mm after 7 days of culture and a phosphorus solubilization content of 49.67 mg·L-1 after 144 hours of incubation.On nutrient agar(NA)plate,colonies of strain G13-2 appeared white,round,moist,and slightly raised.The strain utilized glucose,raffinose,L-rhamnose monohydrate,D-cellobiose,sorbitol,and glycerol,and tested positive for catalase activity,methyl red reaction,and fructan production.Phylogenetic analysis based on 16S rDNA and gyrB gene sequences demonstrated that strain G13-2 clustered distinctly with Klebsiella aerogenes,confirming its classification as K.aerogenes.Strain G13-2 displayed antibacterial activity against Ralstonia pseudosolanacearum isolated from tomato,tobacco,pepper,sweet potato,and ginger,and could produce siderophores.These findings demonstrated that K.aerogenes strain G13-2 possesses dual functionalities of phosphorus solubilization and antibacterial activity against R.pseudosolanacearum,highlighting its potential application prospects.关键词
叶际细菌/解磷/青枯菌/产气克雷伯氏菌/嗜铁素Key words
phyllotropic bacteria/phosphorus solubilizing/Ralstonia pseudosolanacearum/Klebsiella aerogenes/siderophores引用本文复制引用
刘玉兰,吴金梅,邓秀月,杨雯,邱嘉林,蔡学清..一株具解磷功能细菌菌株的鉴定及其生物学功能[J].核农学报,2025,39(7):1425-1433,9.基金项目
福建省引导性项目(2022N0004),中国烟草总公司科技计划项目[110202201028(LS-12)],中国烟草总公司福建省公司科技计划项目(2022350000240061),福建农林大学大学生创新创业项目(X202210389141) （2022N0004）
