花生学报2025，Vol.54Issue(2)：125-133,9.DOI:10.14001/j.issn.1002-4093.2025.02.002
花生杂交种AhMITE分子标记及近红外光谱技术的鉴定研究
Identification of Peanut Hybrid AhMITE by Molecular Markers and Near-Infrared Spectroscopy
摘要
Abstract
Hybridization breeding is one of the important methods for peanut variety improve-ment,accurate identification of F1 hybrid is critical to hybridization breeding in peanut.In this study,12 hybrid combinations of F1 generation peanut and 3 hybrid combinations of F2 seeds obtained from self-crossing of the F1 generation were used as materials to identify the true and false heterozygotes of F1 generation peanut by using miniature inverted-repeat transposable element markers and near-infra-red spectroscopy.A total of 10 pairs of AhMITE primers with significantly different bands in the hy-brid parents were used to identify true and false hybrids in 215 individuals of the F1 generation(12 hy-brid combinations).Two pairs of AhMITE primers were used for each hybrid combination to ensure the accuracy of the identification results.According to the parental band type amplified by PCR,a to-tal of 91 true hybrid single plants were identified,and the true hybrid ratio was 42.33％.The remaining three normal oleic × high oleic hybrid combinations were selected using near-infrared spectroscopy to select F2 single seeds with oleic acid content greater than 75％,which are the true hybrid offspring.The identification methods in this study will help to improve the efficiency of peanut variety selection.关键词
花生/AhMITE/分子标记/杂种鉴定/近红外技术Key words
peanut/AhMITE/molecular marker/hybrid identification/near-infrared spectroscopy分类
农业科技引用本文复制引用
毕竞男,迟晓元,樊兆博,殷祥贞,赵旭红,赵健鑫,陈娜,姜骁,马俊卿,许静..花生杂交种AhMITE分子标记及近红外光谱技术的鉴定研究[J].花生学报,2025,54(2):125-133,9.基金项目
山东省自然科学基金(ZR2021QC172,ZR2023QC146) （ZR2021QC172,ZR2023QC146）
新疆维吾尔自治区重大科技专项(2022A02008-3) （2022A02008-3）
泰山学者工程专项(tstp20240523,tsqn202312292) （tstp20240523,tsqn202312292）
财政部和农业农村部:国家现代农业产业技术体系(CARS-13) （CARS-13）
山东省重点研发计划(2024LZGC035) （2024LZGC035）
山东省重点研发计划(乡村振兴科技创新提振行动计划)(2022TZXD0031) （乡村振兴科技创新提振行动计划）
山东省农业科学院农业科技创新工程(CXGC2023F20,CXGC2024F20) （CXGC2023F20,CXGC2024F20）
浙江省数字旱粮重点实验室培育开放课题(2022E10012) （2022E10012）
农业农村部油料作物生物学与遗传育种重点实验室(KF2024007) （KF2024007）
山东省花生研究所基础研究任务青年基金一般任务(CXGC2025C19) （CXGC2025C19）
