空间科学学报2025，Vol.45Issue(2)：517-528,12.DOI:10.11728/cjss2025.02.2024-0144
血管-骨基质交互微流控芯片实验体系的构建及验证
Construction and Validation of Blood Vessel-bone Matrix Interactive Microfluidic Chip Experimental System
摘要
Abstract
Advanced Glycation End Products(AGEs)in bone matrix are the products of non-enzy-matic glycation of glucose and collagen,which are closely related to the mechanism of weightless bone loss.However,how AGEs accumulate in bone matrix remains unclear.The type L microvessels with slower blood flow velocity were increased under microgravity,which may be related to the accumulation of AGEs in bone.To study the effects of flow velocity on the transport of intravascular glucose molecules into bone matrix and the formation of AGEs in bone matrix,a bilayer channel microfluidic chip experi-mental system was constructed to simulate the blood vessel-bone matrix interface in vitro.A self-devel-oped double-injection-pump continuous directional liquid supply system was applied to the chip,and the biocompatibility,stability and interorganizational interactivity of the system were verified by experi-ments.The results show that the fluid stress distribution in the main region of the channel in fabricated chip is uniform,and the flow is laminar.The endothelial cells in microfluidic chips could grow normally after loading high sugar medium of 2.88 mL·min-1 and 0.38 mL·min-1 for 72 h,respectively.The diffu-sion rate of glucose molecules from to the lower collagen channel through the microporous membrane was higher under low flow rate loading than under high flow rate loading,and more AGEs generated in collagen.The experimental system constructed in this paper has excellent biocompatibility,long-term operational stability and interorganizational interactivity,which lays a technical foundation for further in-depth research on biophysical mechanisms related to AGEs accumulation in bone matrix,and has the potential to be applied to space life science research.关键词
晚期糖基化终末产物/失重性骨丢失/血液流速/微流控芯片/连续定向供液系统Key words
Advanced glycation end products/Weightless bone loss/Blood flow velocity/Microfluidic chip/Continuous directional fluid supply system分类
航空航天引用本文复制引用
刘丛锦,周浩翔,魏栋苹,孙联文,樊瑜波,杨肖..血管-骨基质交互微流控芯片实验体系的构建及验证[J].空间科学学报,2025,45(2):517-528,12.基金项目
中央高校基本科研业务费(JKF-20240765,JKF-20240586),载人空间站工程空间科学与应用项目(KJZ-YY-NSM0504)和国家自然科学基金区域联合基金项目(U20A20390)共同资助 （JKF-20240765,JKF-20240586）
