林业工程学报2025，Vol.10Issue(3)：77-85,9.DOI:10.13360/j.issn.2096-1359.202406021
酸性和碱性氨基酸基低共熔溶剂对酶解效果的影响
The effect of acidic and alkaline amino acid-based deep eutectic solvents on enzymatic hydrolysis efficiency
摘要
Abstract
The comprehensive utilization of bamboo biomass is a vital strategy for maximizing the high-value use of agricultural and forestry waste in China and advancing carbon cycling.As a fast-growing and renewable resource,bamboo holds considerable promise for sustainable development.Deep eutectic solvents,emerging as innovative pretreatment reagents,offer notable advantages,including designability and reusability.These solvents can be tailored to specific applications,making them versatile tools in biomass processing.Applying these solvents to the pretreatment of bamboo biomass can improve the separation efficiency of its three major components:cellulose,hemicellulose,and lignin,and facilitate their individual utilization.This study utilized ethylene glycol and L-arginine(E-Arg)to prepare an alkaline deep eutectic solvent(DES)system,achieving effective separation of carbohydrates and lignin in lignocellulosic biomass.The results indicated that,under pretreatment conditions of 120℃for 3 h,the DES system selectively and efficiently dissolved the lignin,achieving a removal rate of 78.30%.Meanwhile,the majority of hemicellulose and cellulose were preserved,ensuring their availability for subsequent enzymatic hydrolysis processes to produce monosaccharides.The study further examined the effects of solid-liquid ratio,reaction temperature,and reaction time of E-Arg DES pretreatment on the separation efficiency of biomass components and enzymatic hydrolysis yield.The physicochemical properties of the solid fraction rich in cellulose were characterized and analyzed using the scanning electron microscopy,Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD),and other techniques.These advanced analytical methods provided detailed insights into the structural changes and composition of the pretreated biomass.The experimental results demonstrated that the pretreatment process increased the content of glucan and xylan in the enzymatic substrate,enhancing their binding with enzymes and improving the enzymatic digestion.Under optimal conditions,the enzymatic digestion of glucan and xylan reached 77.55%and 85.90%,respectively.This significant improvement in enzymatic digestion rates highlights the effectiveness of the DES pretreatment in enhancing the accessibility of biomass components to enzymatic action.Analysis of the physicochemical properties of the recovered lignin showed that the lignin sample exhibited high purity.After the E-Arg DES pretreatment,the lignin recovery percentage reached 65.20%,indicating that the selective removal of lignin by E-Arg DES in bamboo pretreatment is related to the improvement of subsequent enzymatic hydrolysis efficiency.The high purity of the recovered lignin suggests its potential for various high-value applications,such as in the production of bio-based materials and chemicals.Overall,this study demonstrates the potential of using DES systems for the efficient pretreatment of bamboo biomass,contributing to the sustainable utilization of renewable resources and the advancement of green chemistry.关键词
生物质精炼/低共熔溶剂/酶解糖化/回收木质素Key words
biomass refining/deep eutectic solvent/enzymatic hydrolysis/recovered lignin分类
化学化工引用本文复制引用
孟繁阳,冯莹莹,徐晨,潘晖..酸性和碱性氨基酸基低共熔溶剂对酶解效果的影响[J].林业工程学报,2025,10(3):77-85,9.基金项目
国家重点研发计划(2023YFD2201400). （2023YFD2201400）
