中南医学科学杂志2025，Vol.53Issue(3)：377-382,6.DOI:10.15972/j.cnki.43-1509/r.2025.03.001
基于生物信息学探究敲低BASP1对HEK293细胞自噬的影响
Exploring the effect of knocking down BASP1 on autophagy in HEK293 cells based on bioin-formatics
摘要
Abstract
Aim To explore the effect of knocking down BASP1 on autophagy in human embryonic kidney 293(HEK293)cells based on bioinformatics.Methods The correlation between BASP1 and human diseases was analyzed using bioinformatics.BASP1-interacting protein were screened by ultra-high performance liquid chromatography-mass spectrometry,and the enriched path-ways of related genes were analyzed by using KEGG and GO.After cells were divided into experimental groups,the autophagy related protein levels of LC3,p62,and ulk1 in each group were detected by immunoblotting,and changes in autophagic structure of cells in each group were observed by using transmission electron microscopy.Results Bioinformatics analysis indicates that the expression level of BASP1 is associated with diseases such as Alzheimer's disease and tumors.The proteins interacting with BASP1 are mainly enriched in the pathways of neurodegenerative diseases and the process of cellular autophagy.The cell experiment results showed that knocking down BASP1 increased the proportion of autophagic lysosomes and the level of ulk1 protein(P＜0.05);After treatment with the autophagy flow blocker chloroquine,knocking down BASP1 resulted in a decrease in the proportion of autophagosomes,an increase in the proportion of autophagosomes,and an increase in the expression level of LC3 protein(P＜0.05).Conclusion Knockdown of BASP1 can enhance the autophagic flow of HEK293 cells,revealing its association with cellular autophagy.关键词
BASP1/自噬/神经退行性疾病/生物信息学Key words
BASP1/autophagy/neurodegenerative diseases/bioinformatics分类
医药卫生引用本文复制引用
陈雯,伍晓棋,王丹玲..基于生物信息学探究敲低BASP1对HEK293细胞自噬的影响[J].中南医学科学杂志,2025,53(3):377-382,6.基金项目
国家自然科学基金面上项目(30971469) （30971469）
湖南省科技创新重点工程项目(2021SK1014) （2021SK1014）
