中国海洋大学学报（自然科学版）2025，Vol.55Issue(6)：21-31,11.DOI:10.16441/j.cnki.hdxb.20240160
墨瑞鳕养殖尾水对雨生红球藻生长和藻际细菌多样性的影响
Effects of Aquaculture Wastewater of Murray Cod on the Growth of Haematococcus pluvialis and Diversity of Phycosphere Bacteria
摘要
Abstract
In order to verify the feasibility of culturing Haematococcus pluvialis in the aquaculture wastewater of Murray cod(Macculochella peelii peelii),a 12-day culture experiment was carried out with MCM medium as the control.The growth and physiological characteristics and nitrogen assimilation enzyme activities of H.pluvialis,nutrient concentrations and bacterial community structure of phycosphere in the wastewater were analyzed.The results showed that H.pluvialis efficiently removed NO3--N from the aquaculture wastewater of Murray cod,and the removal rate reached 99.94％in 12 days.The aquaculture wastewater of Murray cod promoted the growth of H.pluvialis;the relative growth rate of H.pluvialis significantly increased(P＜0.05).The biomass and astaxanthin production of H.pluvialis significantly increased with the increase of wastewater content(P＜0.05)while chlorophyll a concentration,soluble sugar content and soluble protein content significantly decreased with the increase of aquaculture wastewater content(P＜0.05).The aquaculture wastewater of Murray cod had a high N∶P ratio(N∶P＞100∶1),and the deficiency of PO43--P may have limited the N absorption of H.pluvialis.In addition,we found that autotrophic denitrifying bacterium Silanimonas was dominant as a genus in wastewater treatment group,and its abundance increased with wastewater content reduction.These bacteria may play a key role in NO3--N removal.Our findings provided basic data for the construction of microalgae-bacteria co-culture system for wastewater purification.关键词
养殖尾水/雨生红球藻/硝态氮/藻际细菌Key words
aquaculture wastewater/Haematococcus pluvialis/nitrate nitrogen/phycosphere bacteri-um分类
生物学引用本文复制引用
凌滨峰,刘岩,高旭,宫庆礼,王巧晗..墨瑞鳕养殖尾水对雨生红球藻生长和藻际细菌多样性的影响[J].中国海洋大学学报（自然科学版）,2025,55(6):21-31,11.基金项目
国家重点研究发展计划项目(2024YFD2400300) （2024YFD2400300）
山东省重点研发计划重大科技创新工程项目(2022ZLGX01)资助 Supported by the National Key Research and Development Program of China(2024YFD2400300) （2022ZLGX01）
the Key Scientific and Technological Innovation Project of Shandong Province's Major Research and Development Program(2022ZLGX01) （2022ZLGX01）
