中国海洋大学学报（自然科学版）2025，Vol.55Issue(6)：32-39,8.DOI:10.16441/j.cnki.hdxb.20240204
纳米塑料对斑马鱼幼鱼行为和能量代谢的影响
Effects of Nanoplastics on Behavior and Energy Metabolism of Zebrafish Larvae
摘要
Abstract
Microplastics and nanoplastics have become important factors threatening the health of aquatic organisms.In this study,zebrafish(Danio rerio)larvae were used as model organisms to investigate the short-term effects of nanoplastic exposure on the behavior and metabolism of juvenile fish.The larvae were exposed to environments containing fluorescent polystyrene nanoplastics(PSNP),25 nm and 80 nm in diameter,respectively,to detect nanoplastic accumulation and assess their effects on zebrafish larval behavior and metabolism.The results showed that after 9 hours of treatment,25 nm PSNP specifically accumulated in the brain tissues of zebrafish while 80 nm PSNP tended to accumulate on the surface of zebrafish larvae.The 25 nm PSNP significantly inhibited the touch-evoked reponse of zebrafish larvae;and significantly reduced the contents of glucose thus inhibited glycolysis and the tricarboxylic acid cycle metabolic pathways.Additionally,25 nm PSNP significantly reduced the level of adenosine triphosphate(ATP)and reduced nicotinamide adenine dinucleotide(NADH).These results indicated that bioaccumulation of nanoplastics is related to their particle size,and nanoplastics significantly inhibit the energy metabolism of zebrafish larvae.Our findings provided important insights for assessing the impact of nanoplastic pollutant on the behavior and metabolism of juvenile fish.关键词
纳米塑料/斑马鱼/行为/糖代谢/三羧酸循环Key words
nanoplastic/zebrafish/behavior/sugar metabolism/tricarboxylic acid cycle分类
环境科学引用本文复制引用
白艳,余皖娟,赵吉灿,杨恒,刘成栋..纳米塑料对斑马鱼幼鱼行为和能量代谢的影响[J].中国海洋大学学报（自然科学版）,2025,55(6):32-39,8.基金项目
国家自然科学基金项目(32002365)资助 Supported by the National Natural Science Foundation of China(32002365) （32002365）
