中国海洋大学学报（自然科学版）2025，Vol.55Issue(6)：40-49,10.DOI:10.16441/j.cnki.hdxb.20240102
以原位负载AgNPs的策略制备抗菌化硅藻生物硅的研究
Preparing Antibacterial Diatom Biosilicon by in situ Loading AgNPs
摘要
Abstract
This study was carried out to reduce bacterial attachment to the surface of diatom biosilica through antibacterial treatment,and avoid the potential risk of wound infection.The reduction method of in situ loading AgNPs onto diatom biogenic silicon was used to prepare the antibacterial diatom biogenic silica DB-AgNPs.Silver nitrate was used as the silver source,and polyvinylpyrrolidone(PVP)as the dispersant protector.The effects of different reducing agents and initial silver ion addition on loading AgNPs onto DB-AgNPs were studied with the Ag release ability,silver loading capacity and antibacterial effect of DB-AgNPs explored.Sodium borohydride was used as a reducing agent to achieve efficient loading of DB-AgNPs onto AgNPs.The silver loading of DB-AgNPs was related to the initial amount of silver ions added.With the increase of initial amount of silver ions added,the silver loading of DB-AgNPs increased correspondingly.When the initial silver ion was 16.70 mg/mL,the silver loading of DB-AgNPs3 maximized at 22.57％.The Ag+release experiment showed that DB-AgNPs exhibited the ability to slowly release Ag+.In the antibacterial experiment,DB-AgNPs showed effective antibacterial ability against E.coli,S.aureus and S.mutans with inhibition rate greater than 90％.A novel antibacterial diatom biosilicon DB-AgNPs was prepared by in situ loading AgNPs.DB-AgNPs have a high silver loading capacity,which showed efficient and extensive antibacterial effects by releasing Ag+.Its application can extend our trials of using diatomaceous biosilicon as the antibacterial biomaterials.关键词
硅藻生物硅/银纳米颗粒/原位负载/抗菌性能Key words
diatom biosilica/silver nanoparticle/in situ loading/antimicrobial property分类
口腔医学引用本文复制引用
刘金凤,孙小杰,车晟廷,孙赟基,陈西广..以原位负载AgNPs的策略制备抗菌化硅藻生物硅的研究[J].中国海洋大学学报（自然科学版）,2025,55(6):40-49,10.基金项目
国家自然科学基金项目(U22A20588) （U22A20588）
三亚市创新专项(2022KJCX57) （2022KJCX57）
崂山实验室专项(12-04) （12-04）
海南省教育厅支持项目(Hnjg2024-276)资助 Supported by the National Natural Science Foundation of China(U22A20588) （Hnjg2024-276）
the Sanya Science and Technology Project(2022KJCX57) （2022KJCX57）
the Laoshan Laboratory Science and Technology Innovation Project(12-04) （12-04）
the Project Supported by the Education Department of Hainan Province(Hnjg2024-276) （Hnjg2024-276）
