中国海洋大学学报（自然科学版）2025，Vol.55Issue(6)：50-59,10.DOI:10.16441/j.cnki.hdxb.20230167
烷基有机磷酸酯对浮游植物生长的影响及作用机制研究
Effect of Alkyl Organophosphorus Flame Retardants on the Growth of Phytoplankton and Associated Mechanism
摘要
Abstract
To address the question,which is the mechanism that alkyl organophosphorus ester(OPEs)-toxicity on phytoplankton growth is remain largely unknown.This study chose tributyl phos-phate(TnBP)was chosen as a representative of alkyl OPEs to investigate its effects on the growth of marine phytoplankton,Phaeodactylum tricornutum,and to reveal in deep its potential toxic mechanism based on the changes of key genes of photosynthesis and energy metabolism,with a view to establishing a theoretical reference for the model of the toxic effect of alkyl OPEs on phytoplankton.The results showed that the population growth of Phaeodactylum tricornutum was significantly inhibited when ex-posed to TnBP at concentrations of 1.5(the maximum level in aquatic environment),3.0 and 4.5 μmol/L,and the intracellular total organic carbon,chlorophyll,and carotenoids contents were also reduced.The chlorophyll fluorescence detection showed that TnBP reduced the maximum photochemical rate Fv/Fm of photosystem Ⅱ and non-photochemical quenching capacity in P.tricornutum,which indicated that photosynthesis was significantly inhibited.Real-time fluorescence quantitative PCR re-sults showed that TnBP down-regulated the expression of two key genes for chlorophyll synthesis,which encoded coproporphyrinogen Ⅲ oxidase and the D subunit of magnesium chelate.Meanwhile,the expression level of two genes(ZEP1 and ZEP3)that encode zeaxanthin epoxidase,a key enzyme for lutein synthesis,also showed a declining trend.It indicated the ability of light-capture and photoprotec-tion of algal cells impaired,which led to a decrease in photosynthetic efficiency and a reduction in organ-ic carbon content.Additionally,it was found that the genes encoding succinate dehydrogenase SDH2 and glyceraldehyde-3-phosphate dehydrogenase GAPC1 were down-regulated,which was suggested that this may lead to an nttenuation of glycolytic pathway and tricarboxylic acid cycle within the algal cells.Therefore,TnBP interfered with intracellular substance synthesis and accumulation by phytoplankton photosynthesis and energy metabolism,which ultimately inhibited cell division and growth.The results of this study can provide a theoretical reference for the establishment of the toxic effect model of alkyl OPEs on phytoplankton.关键词
烷基有机磷酸酯/浮游植物/生长抑制/光合作用/能量代谢Key words
alkyl organophosphate esters/phytoplankton/growth inhibition/photosynthesis/energy metabolism分类
环境科学引用本文复制引用
童欣,唐学玺,刘倩,王承敏,张鑫鑫..烷基有机磷酸酯对浮游植物生长的影响及作用机制研究[J].中国海洋大学学报（自然科学版）,2025,55(6):50-59,10.基金项目
青岛海洋科学与技术试点国家实验室"十四五"重大项目(LSKJ202203900)资助 Supported by the Pilot National Laboratory of Marine Science and Technology(Qingdao)"14th Five-Year Plan"Major Project(LSKJ202203900) （LSKJ202203900）
