日用化学工业（中英文）2025，Vol.55Issue(5)：581-590,10.DOI:10.3969/j.issn.2097-2806.2025.05.006
白花蛇舌草提取液对日光性皮炎小鼠的保护作用及机制研究
Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis
摘要
Abstract
The paper aimed to study the antioxidant and anti-inflammatory effects of Hedyotis diffusa extract on mice with ultraviolet B(UVB)-induced solar dermatitis.KM mice were subjected to UVB irradiation for 7 days,which were also treated with topical application of 1%and 2%Hedyotis diffusa extract.Hematoxylin-eosin(HE)staining,and picric acid-acidic magenta method(VG)staining were performed to observe the state of mouse skin.The levels of oxidative stress indicators NOS,NO,T-SOD,and inflammation indicators TNF-α,IL-6,and IL-1β were detected by biochemistry and immunohistochemistry,and the mRNA or protein levels of Nrf2,Keap1,HO-1,NQO1,GCLC,NF-κB,HMGB1 and p65 were detected by qRT-PCR and western blotting in skin tissues.Compared with the UVB group,the degree of skin damage in mice in the 1%and 2%Hedyotis diffusa extract groups is improved,with collagen fibers aligned,collagen density increased,NOS and NO levels decreased,T-SOD levels increased,TNF-α,IL-6,IL-1β expression levels decreased,mRNA expression levels of Nrf2,Keap1,HO-1,NQO1,and GCLC in skin tissue increased,NF-κB,HMGB1 mRNA expression levels decreased,the relative protein expression levels of Nrf2,Keap1,HO-1,NQO1,GCLC,and p65 in skin tissues increased,and the protein expression level of HMGB1 decreased.It can be seen that Hedyotis diffusa extract can ameliorate UVB-mediated solar dermatitis in mice,and its antioxidant and anti-inflammatory mechanism may be related to Nrf2 and NF-κB signaling pathway.关键词
白花蛇舌草提取液/紫外线B/日光性皮炎/抗氧化/抗炎Key words
Hedyotis diffusa extract/ultraviolet B/solar dermatitis/antioxidant/anti-inflammatory分类
化学工程引用本文复制引用
马悦文,李小静,黄雪锋,陈贤,王春丽..白花蛇舌草提取液对日光性皮炎小鼠的保护作用及机制研究[J].日用化学工业（中英文）,2025,55(5):581-590,10.基金项目
国家科技重大专项(2018ZX09735002) （2018ZX09735002）
