日用化学工业（中英文）2025，Vol.55Issue(5)：601-610,10.DOI:10.3969/j.issn.2097-2806.2025.05.008
芹菜素和原花青素舒缓敏感肌肤的作用机制
Mechanism of the action of apigenin and proanthocyanidins in alleviating sensitive skin
摘要
Abstract
To investigate the mechanisms of apigenin(API)and proanthocyanidins(PC)in soothing sensitive skin(SS),a mast cell degranulation model was established by stimulating RBL-2H3 cells with the calcium ionophore A23187.Based on the combinatorial experiments,it was found that when API and PC were combined at the molar ratios of 4∶1 and 2∶1,they exhibited the antagonistic effects on histamine release(combination index CI>1);when they are combined at the molar ratios of 1∶1,1∶2 or 1∶4,they showed the synergistic effects on histamine release(CI<1).Among them,the combination of API and PC at a molar ratio of 1∶1 showed the better potent synergistic antihistamine release effect(CI=0.70).Histamine is a hallmark of the mast cell degranulation,consequently,the combination of API and PC at a molar ratio of 1∶1 yields the better efficiency in inhibiting the mast cell degranulation with the lowest IC50 value.Compared to the utilization of API or PC alone,the IC50 value was reduced by 11.150 and 6.503µmol/L,respectively.Compared to the positive control paeonol(PA),the treatment with the combination significantly reduced the β-hex secretion,decreased the F-actin cytoskeleton rearrangement,and markedly suppressed the release of TNF-α,IL-4,and MCP-1.Further studies on the signaling pathways related to the mast cell degranulation indicated that the combination effectively inhibited the intracellular Ca2+influx and significantly suppressed the phosphorylation of calmodulin-dependent protein kinase(CaMK)and phospholipase C/protein kinase C(PLC/PKC).In summary,the combination of API and PC at a molar ratio of 1∶1 exhibited the better synergistic antagonistic effect on the histamine release,inhibited the mast cell degranulation model activation by reducing Ca2+influx and inhibiting the activation of Ca2+/CaMK and PLC/PKC pathways,stabilized the cell membranes,regulated the inflammatory factor secretion,and exerted an effect in alleviating sensitive skin.关键词
芹菜素/原花青素/肥大细胞/敏感肌/舒缓Key words
apigenin/proanthocyanidins/mast cell/sensitive skin/soothing分类
化学工程引用本文复制引用
魏来娇,邓榕榕,郑诗倩,李珞炫,王科证,沈志滨..芹菜素和原花青素舒缓敏感肌肤的作用机制[J].日用化学工业（中英文）,2025,55(5):601-610,10.基金项目
国家重点研发计划重点专项项目(2018YFC1707100) （2018YFC1707100）
