日用化学工业（中英文）2025，Vol.55Issue(5)：633-639,7.DOI:10.3969/j.issn.2097-2806.2025.05.012
蒲公英多酚的抑菌和抗炎活性研究
Study on the antibacterial activity and anti-inflammatory activity of dandelion polyphenols
摘要
Abstract
The study aimed to reveal the antibacterial activity of dandelion polyphenols(DP)against common skin pathogens and anti-inflammatory activity.In this study,six common skin pathogens were treated with different concentrations of dandelion polyphenols for 24 hours,and the minimum inhibitory concentration(MIC)and the diameter of bacteriostatic zone were measured.CCK-8 kit was used to detect the toxicity of dandelion polyphenols to RAW264.7 cells.RAW264.7 cells were divided into Blank group,LPS group,LPS+10 DP group,LPS+50 DP group,LPS+100 DP group,LPS+200 DP group,LPS+400 DP group,LPS+800 DP group and LPS+1 600 DP group.The cells were treated with saline,LPS(1 μg/mL)and different concentrations of dandelion polyphenols for 24 hours.The content of NO was measured by visible spectrophotometry.The mRNA levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-6 were detected by RT-qPCR.The phosphorylation levels of NF-kappa B(NF-κB)p65 and p38 mitogen activated protein kinase(MAPK)were detected by Western blotting.The nuclear translocation of NF-κB p65 was observed by immunofluorescence staining.The results show that the MIC of dandelion polyphenols against Staphylococcus aureus,Staphylococcus epidermidis,Escherichia coli,Pseudomonas aeruginosa,Proteus vulgaris and Cutibacterium acnes are 400,400,400,800,800 and 400 μg/mL,respectively.When the concentration of dandelion polyphenols is over 800 μg/mL,all the six pathogens have obvious bacteriostatic circles(P<0.001).The concentration of dandelion polyphenols in the range of 10 to 1 600 μg/mL is non-toxic to RAW264.7 cells(P>0.05).Compared with LPS group,NO content and relative levels of iNOS,COX-2,TNF-α,IL-1β and IL-6 mRNA in RAW264.7 cells in LPS+100 DP group,LPS+200 DP group,LPS+400 DP group,LPS+800 DP group and LPS+1 600 DP group decrease(P<0.05),the relative phosphorylation levels of NF-κB p65 and p38 MAPK decrease(P<0.05),and the nuclear translocation of NF-κB p65 decreases.The above results indicate that dandelion polyphenols have antibacterial activity against common skin pathogens,and can inhibit LPS-induced RAW264.7 cell inflammation and the activation of NF-κB p65 and p38 MAPK signaling pathways.关键词
蒲公英多酚/皮肤常见病原菌/抑菌/抗炎/核因子-κB/p38丝裂原活化蛋白激酶Key words
dandelion polyphenols/common skin pathogens/bacteriostasis/anti-inflammation/nuclear factor-κB/p38 mitogen-activated protein kinase分类
化学化工引用本文复制引用
谢诗雅,刘明巍,肖雷,胡绍霖..蒲公英多酚的抑菌和抗炎活性研究[J].日用化学工业（中英文）,2025,55(5):633-639,7.基金项目
信阳职业技术学院校级科研基金(LXQN231102) （LXQN231102）
