日用化学工业（中英文）2025，Vol.55Issue(5)：668-676,9.DOI:10.3969/j.issn.2097-2806.2025.05.016
化妆品微生物ATP生物荧光增幅检测技术的双培养体系构建及可行性研究
Establishment and feasibility study of a dual enrichment system for amplified ATP bioluminescence microbial detection assay in cosmetics
李婷 1马紫英 1刘吉泉 2崔生辉 3景宇 1白飞荣 1姚粟1
作者信息
- 1. 中国食品发酵工业研究院有限公司,北京 100015
- 2. Procter & Gamble International Operations,新加坡 138547
- 3. 中国食品药品检定研究院,北京 100050
- 折叠
摘要
Abstract
To improve the microbial detection efficiency in cosmetics,an amplified ATP bioluminescence 24 h assay with dual enrichment system was established.Meanwhile,growth promotion test of an enrichment medium and product suitability test was carried out.11 of slow-growing fungi,yeasts and bacteria were selected as representative strains,and the key parameters such as culture temperature and enrichment medium formula were optimized.The results show that the enrichment system that promoted fungal growth is TLE medium incubated at 25℃,and the bacterial enrichment system is MTAT medium incubated at 32.5℃.32 representative strains relevant to raw materials and production environment of cosmetics are collected and tested.They all grow well in the enrichment system,and the results of amplified ATP bioluminescence 24 h assay are all positive.Subsequently,six kinds of cosmetics with different functional claims,use parts,production dosage forms and use methods are selected and inoculated with six reference strains recommended by Chinese Pharmacopoeia(2020)for product suitability test.The background values of the tested cosmetics meet the requirements of the detection method.Results of spiking test show that all the samples tested are positive.Therefore,amplified ATP bioluminescence 24 h assay has a promising application prospect in the rapid screening of microorganisms in cosmetic fields.关键词
ATP生物荧光增幅/化妆品/双培养体系/促生长能力/产品适用性Key words
amplified ATP bioluminescence/cosmetics/dual enrichment system/growth promotion test/product suitability test分类
化学化工引用本文复制引用
李婷,马紫英,刘吉泉,崔生辉,景宇,白飞荣,姚粟..化妆品微生物ATP生物荧光增幅检测技术的双培养体系构建及可行性研究[J].日用化学工业（中英文）,2025,55(5):668-676,9.
