中度嗜盐菌NY-011对枯草芽孢杆菌KK定殖稳定性提高的分子机理OA北大核心

To ensure the stabilize rhizosphere growth-promoting function of Bacillus subtilis,this research ex-plored co-culture with the moderately halophilic bacterium NY-011(Halomonas sp.NY-011),focusing on its effects on biofilm formation,soil colonization,and the soil microbial community structure.The results showed that the dry weight of the KK biofilm increased by 4.8%following the addition of NY-011 extract,and the surface of the biofilm became denser and rougher.The expression of genes encoding EPS exopolysac-charides,as well as tasA and tapA,increased by 5～10 fold.Additionally,the expression of KinD kinase and Spo0A regulator,both involved in biofilm signal transduction,increased by 21%and 33%,respectively,while the expression of the biofilm synthesis inhibitor gene AbrB decreased by 30%.The addition of NY-011 improved the soil colonization rate of KK by 65%,and significantly enhanced the richness and evenness of the soil microbial community,as indicated by higher Chao1,Observed species,Shannon,and Simpson indices.These results suggest that the inclusion of moderately halophilic bacteria can significantly enhance the coloni-zation stability of Bacillus subtilis KK and improve soil microecological balance.