四川大学学报（自然科学版）2025，Vol.62Issue(3)：713-720,8.DOI:10.19907/j.0490-6756.240247
香叶醇对耐药大肠埃希菌感染小鼠肾损伤的保护机制
Protective mechanism of geraniol on kidney injury caused by multidrug-resistant Escherichia coli in mice
摘要
Abstract
To investigate the protective effect and underlying mechanisms of geraniol against kidney injury in-duced by multidrug-resistant Escherichia coli in mice,we hypothesizing that geraniol alleviates renal patho-logical damage by modulating antioxidant and anti-inflammatory signaling pathways.Multidrug-resistant Esch-erichia coli bacterial suspension was intraperitoneally injected into mice,the mouse bacterial infection model was established followed by intramuscular injection of 0.092 g/kg geraniol once daily for three consecutive days,and mouse kidneys were collected on the 4th day.Histopathological changes in renal histology after in-fection were observed by the H&E staining method.The relative mRNA expression levels of Nrf2,HO-1 and GPX4 in the kidney were measured by qPCR,and the contents of LDH and SOD in the kidney were de-tected by assay kits.Immunohistochemistry and Western blot were used to detect the protein expressions of Nrf2,HO-1,and GPX4.Multidrug-resistant E.coli infection led to medullary congestion,dilated collecting ducts,multifocal congestion,and significant inflammatory cell infiltration in kidneys.In the geraniol-treated group,inflammatory cell infiltration was reduced,and pathological damage to the glomeruli and renal tubules was alleviated.Additionally,LDH activity was significant decreased,while SOD activity was increased(P<0.05).Geraniol significantly upregulated the mRNA expression of Nrf2,HO-1 and GPX4(P<0.05)and enhanced the protein expression of Nrf2 and GPX4(P<0.05),though HO-1 protein expression remained unchanged.These findings suggest that geraniol effectively mitigates kidney injury caused by multidrug-resistant E.coli,potentially through activation of the Nrf2/HO-1/GPX4 signaling pathway,which alleviates inflammatory and oxidative stress damage.This highlights its potential for the development of drugs targeting multidrug-resistant E.coli infections.关键词
多重耐药/香叶醇/大肠埃希菌/Nrf2/HO-1/GPX4信号通路/肾损伤Key words
Multidrug resistance/Geraniol/Escherichia coli/Nrf2/HO-1/GPX4 signaling pathway/Kidney damage分类
医药卫生引用本文复制引用
王雨珍,宁溢潇,代敏..香叶醇对耐药大肠埃希菌感染小鼠肾损伤的保护机制[J].四川大学学报（自然科学版）,2025,62(3):713-720,8.基金项目
四川省科技厅重点研发计划(2024YFFK0090) （2024YFFK0090）
四川省中医药管理局创新团队项目(2023ZD02) （2023ZD02）
国家自然科学基金项目(82472328). （82472328）
