香叶醇对耐药大肠埃希菌感染小鼠肾损伤的保护机制OA北大核心

To investigate the protective effect and underlying mechanisms of geraniol against kidney injury in-duced by multidrug-resistant Escherichia coli in mice,we hypothesizing that geraniol alleviates renal patho-logical damage by modulating antioxidant and anti-inflammatory signaling pathways.Multidrug-resistant Esch-erichia coli bacterial suspension was intraperitoneally injected into mice,the mouse bacterial infection model was established followed by intramuscular injection of 0.092 g/kg geraniol once daily for three consecutive days,and mouse kidneys were collected on the 4th day.Histopathological changes in renal histology after in-fection were observed by the H&E staining method.The relative mRNA expression levels of Nrf2,HO-1 and GPX4 in the kidney were measured by qPCR,and the contents of LDH and SOD in the kidney were de-tected by assay kits.Immunohistochemistry and Western blot were used to detect the protein expressions of Nrf2,HO-1,and GPX4.Multidrug-resistant E.coli infection led to medullary congestion,dilated collecting ducts,multifocal congestion,and significant inflammatory cell infiltration in kidneys.In the geraniol-treated group,inflammatory cell infiltration was reduced,and pathological damage to the glomeruli and renal tubules was alleviated.Additionally,LDH activity was significant decreased,while SOD activity was increased(P<0.05).Geraniol significantly upregulated the mRNA expression of Nrf2,HO-1 and GPX4(P<0.05)and enhanced the protein expression of Nrf2 and GPX4(P<0.05),though HO-1 protein expression remained unchanged.These findings suggest that geraniol effectively mitigates kidney injury caused by multidrug-resistant E.coli,potentially through activation of the Nrf2/HO-1/GPX4 signaling pathway,which alleviates inflammatory and oxidative stress damage.This highlights its potential for the development of drugs targeting multidrug-resistant E.coli infections.