兽类学报2025，Vol.45Issue(3)：283-294,12.DOI:10.16829/j.slxb.150940
低氧环境下高原鼢鼠及SD大鼠肺和骨骼肌中SP-A的表达模式
The expression patterns of surfactant protein A(SP-A)in lung and skele-tal muscle in SD rat and plateau zokor(Eospalax baileyi)under the hy-poxia environments
摘要
Abstract
Pulmonary surfactant protein A(Surfactant protein A,SP-A)is the most abundant pulmonary surfactant pro-tein,encoded and synthesized by the SFTPA gene.SP-A is primarily synthesized in the lung tissue of mammals and is al-so expressed in tissues such as skeletal muscle.SP-A has functions including reducing pulmonary surface tension,en-hancing pulmonary gas exchange,regulating immunity,clearing pathogens,and reducing inflammatory responses.In this study,after performing transcriptome sequencing analysis on the liver,lung,skeletal muscle,small intestine,colon,and adipose tissues from SD rats(2 250 m and 3 700 m)and plateau zokors(2 700 m and 3 700 m)at different altitudes;the expression levels of SFTPA gene in skeletal muscle and lung tissues of the two species were compared and analyzed by qRT-PCR;the expression levels of SP-A in skeletal muscle,lung tissue and serum of the two species were compared and analyzed by Western Blotting.The transcriptome analysis revealed that with increasing altitude,the expression of the SFTPA gene in the skeletal muscle of SD rats were significantly up-regulated,while in the skeletal muscle of plateau zokors,there were no significant difference in its expression;the qRT-PCR results showed that with increasing altitude and decreasing environmental oxygen levels,the expression of SFTPA in the skeletal muscle of SD rats increased,where-as in the skeletal muscle of plateau zokors,the expression of SFTPA significantly decreased.Furthermore,the expression of SFTPA in the lung tissues of both SD rats and plateau zokors were significantly decreased;the Western Blotting results showed that with increasing altitude and decreasing environmental oxygen levels,the expression levels of SP-A in the skeletal muscle,serum,and lung tissues of both SD rats and plateau zokors were significantly increased.In conclusion,in a high-altitude hypoxia environment,the transcriptional expression of the SFTPA gene in the lung tissues of SD rats and plateau zokors were significantly decreased.However,the content of SP-A in their lung tissues not only did not de-crease but significantly increased.This may be due to SP-A synthesized and secreted by skeletal muscle being transport-ed to the lung tissues via the bloodstream,representing an important mechanism for SD rats and plateau zokors to adapt to hypoxia environments.关键词
肺表面活性物质/肺表面活性蛋白A/高原低氧/SD大鼠/高原鼢鼠Key words
Pulmonary surfactant(PS)/Surfactant protein A(SP-A)/High-altitude hypoxia/SD rat/Plateau zokor(Eo-spalax baileyi)分类
生物学引用本文复制引用
蒲小燕,戴重阳,王雅轩,魏登邦..低氧环境下高原鼢鼠及SD大鼠肺和骨骼肌中SP-A的表达模式[J].兽类学报,2025,45(3):283-294,12.基金项目
第二次青藏高原综合科学考察研究项目(2019QZKK0501) （2019QZKK0501）
青海省自然科学基金(2021-ZJ-904) （2021-ZJ-904）
