兽类学报2025，Vol.45Issue(3)：295-301,7.DOI:10.16829/j.slxb.150902
白血病抑制因子(LIF)过表达对大熊猫骨髓间充质干细胞生长的影响
Effect of overexpression of leukemia inhibitory factor(LIF)on the growth of bone marrow mesenchymal stem cells of giant panda
摘要
Abstract
Leukemia inhibitory factor(LIF)is an important factor regulating cell growth,proliferation and differentia-tion.Bone marrow mesenchymal stem cells(BM-MSCs)have broad application prospects in the repair of tissue damage as well as the treatment of autoimmune diseases,and,as a result,have great potential in the disease treatment and genetic conservation of rare and endangered animals,such as giant pandas.The aim of this study was to construct a giant panda LIF overexpression vector and transfect panda bone marrow mesenchymal stem cells(PDBM-MSCs)with liposome to study the effect of LIF on the growth of PDBM-MSCs.The CDS region of the LIF gene was amplified from the cDNA of PDBM-MSCs,and seamlessly cloned onto the pCDH-CMV-MCS-EF1-GFP+puro overexpression vector to construct the LIF overexpression recombinant plasmid.Lipo3000 was used to transfect recombinant plasmid into PDBM-MSCs.The results showed that PDBM-MSCs expressed green fluorescence 48 h after transfection,and total RNA was extracted and reverse-transcribed into cDNA 72 h after transfection.A q-PCR detection showed that the mRNA expression level of LIF in the LIF overexpression group was significantly higher than that in the negative control group.Moreover,over-expression of LIF could significantly up-regulate the mRNA expressions of CCNB2 and CDK7,and significantly down-regulate the mRNA expressions of apoptosis genes caspase3,P53,P16 and P21.72 h after transfection,flow detection showed that the viability of PDBM-MSCs transfected with LIF overexpression recombinant plasmid was significantly higher than that of the negative control group.In this study,the panda LIF overexpression vector was successfully con-structed and transfected into PDBM-MSCs,which significantly improved cell viability and inhibited apoptosis,indicat-ing that LIF can promote growth and inhibit apoptosis of PDBM-MSCs,laying a foundation for the study of the mecha-nism of LIF in giant pandas.关键词
大熊猫/骨髓间充质干细胞/白血病抑制因子/细胞生长Key words
Giant panda/Bone marrow mesenchymal stem cells/Leukemia inhibitor/Cell growth分类
生物学引用本文复制引用
李非平,张梦诗,王神飞,胡贤彪,刘玉良,侯蓉,刘项宇,蔡开来..白血病抑制因子(LIF)过表达对大熊猫骨髓间充质干细胞生长的影响[J].兽类学报,2025,45(3):295-301,7.基金项目
四川省自然科学基金重点项目(2022NSFSC0010,2024CPB-P02) （2022NSFSC0010,2024CPB-P02）
成都大熊猫繁育研究基地自立课题(2022CPB-B10) （2022CPB-B10）
