饲料研究2025，Vol.48Issue(8)：54-60,7.DOI:10.13557/j.cnki.issn1002-2813.2025.08.010
饲料中添加海藻多糖对加州鲈肠炎治疗作用
Therapeutic effects of adding dietary seaweed polysaccharides on enteritis of Micropterus salmoides
摘要
Abstract
The experiment induced enteritis in Micropterus salmoides by injecting 7.5%2,4-dinitrobenzenesulfonic acid(DNBS),and explored the therapeutic effect of adding seaweed polysaccharides to the compound feed on Micropterus salmoides with enteritis.A total of 120 Micropterus salmoides weighing approximately 25 g are selected and randomly divided into two groups,with three replicates in each group,and 20 fish in each replicate.The D0 group was fed a basal diet,while the D2 group is fed a diet supplemented with 2‰ seaweed polysaccharides.The pre-test period was one week,the formal test period was two weeks.The results showed that compared to the D0 group,the D2 group exhibited no significant difference in growth performance(P>0.05),serum albumin(ALB)levels were significantly increased(P<0.05),and serum interleukin-6(IL-6)levels were significantly decreased(P<0.05).In the anterior intestine,glutathione peroxidase(GSH-Px)and catalase(CAT)activities were significantly increased(P<0.05),while myeloperoxidase(MPO)activity,malondialdehyde(MDA)levels,and endotoxin(LPS)content were significantly reduced(P<0.05).The intestinal villi in the posterior intestine were more intact,and the thickness of the intestinal wall muscle layer was increased.The D2 group significantly downregulated the mRNA expression of pro-inflammatory factors tumor necrosis factor-α(TNF-α)and IL-6(P<0.05),while upregulating the mRNA expression of anti-inflammatory factors interleukin-10(IL-10),hypoxia-inducible factor(HIF),short-chain fatty acid receptor 3(FFAR3),and intestinal epithelial mucin-2(MUCIN-2)(P<0.05).The abundance of certain bacterial genera,including Methyloversatilis,Cetobacterium,Corynebacterium and the pathogenic genus Plesiomonas was significantly reduced while the abundance of the beneficial genus Clostridium was significantly increased.No significant differences were observed in the content of Gram-positive bacteria,Gram-negative bacteria,or potential pathogens in the gut(P>0.05).The study indicates that dietary supplementation with 2‰ seaweed polysaccharides enhances the antioxidant capacity of Micropterus salmoides with enteritis,reduces intestinal inflammation,improves intestinal histomorphology and microbiota structure,and exhibits therapeutic effects against enteritis in Micropterus salmoides.关键词
海藻多糖/肠炎治疗/加州鲈Key words
seaweed polysaccharides/enteritis treatment/Micropterus salmoides分类
水产学引用本文复制引用
孙淼,李鸿伟,姚闽,陈飞扬,王建磊,周萌,林蠡,游翠红..饲料中添加海藻多糖对加州鲈肠炎治疗作用[J].饲料研究,2025,48(8):54-60,7.基金项目
青岛海兴源生物科技有限公司科技服务项目(项目编号:D121222C8) （项目编号:D121222C8）
广东省自然科学基金项目(项目编号:2020A1515011252) （项目编号:2020A1515011252）
