饲料研究2025，Vol.48Issue(8)：133-138,6.DOI:10.13557/j.cnki.issn1002-2813.2025.08.023
液相色谱-串联质谱法测定饲料中8种真菌毒素的不确定度评定
Uncertainty determination of eight kinds of fungal toxins in feed by liquid chromatography-mass spectrometry evaluation
摘要
Abstract
The experiment aimed to evaluate the uncertainty of liquid chromatography-mass spectrometry evaluation in determining the content of eight fungal toxins in feed.According to the standard'Determination of 37 mycotoxins in feeds-liquid chromatography-tandem mass spectrometry(LC-MS/MS)'(NY/T 3803-2020),establish a mathematical model to analyze the sources of uncertainty and evaluate the uncertainty determination.The results showed that the expanded uncertainties of aflatoxin A,aflatoxin B1,B2,G1,G2 and fumarate toxin B1,B2,B3 were 2.43,0.24,0.30,0.47,0.20,5.15,3.98 and 4.50 μg/kg,respectively.The contents of aflatoxin A,aflatoxin B1,B2,G1,G2 and fumarate toxin B1,B2,B3 in the sample were 59.60,5.31,5.07,5.66,5.57,104.62,102.62,108.22 μg/kg(k=2,corresponding to a 95%confidence probability).The study indicates that the main sources of uncertainty are repetitive measurements and standard configuration,which should be controlled in practical work.关键词
液相色谱-串联质谱法/不确定度/真菌毒素/饲料Key words
liquid chromatography-mass spectrometry evaluation/uncertainty/fungal toxin/feed分类
农业科技引用本文复制引用
韩慧,吴远高,李艳美,李芳,粟有志,王兴磊..液相色谱-串联质谱法测定饲料中8种真菌毒素的不确定度评定[J].饲料研究,2025,48(8):133-138,6.基金项目
新疆维吾尔自治区伊犁哈萨克自治州科技计划项目(项目编号:YJC2024A06) （项目编号:YJC2024A06）
新疆维吾尔自治区自然科学基金项目(项目编号:2021D01A188) （项目编号:2021D01A188）
伊犁师范大学污染物化学与环境治理重点实验室开放课题(项目编号:2021HJYB04) （项目编号:2021HJYB04）
新疆维吾尔自治区天山英才基金项目(项目编号:2023TSYCCX0098、2023TSYCJC0061) （项目编号:2023TSYCCX0098、2023TSYCJC0061）
