饲料研究2025，Vol.48Issue(8)：139-144,6.DOI:10.13557/j.cnki.issn1002-2813.2025.08.024
免疫调节八肽检测方法的构建及其稳定性初步研究
Development of detection method for immunomodulatory octapeptin and preliminary study on its stability
摘要
Abstract
The experiment aimed to construct a detection method for immunomodulatory octapeptin and preliminarily investigate its stability under high-temperature and high-humidity conditions.A quantitative detection method for immunomodulatory octapeptin was constructed using high-performance liquid chromatography(HPLC),and determined the properties,moisture content and changes in the content of immunomodulatory octapeptin of the samples under high temperature and high humidity conditions.The results showed that the constructed detection method had a good linear relationship in the range of 0～5 000 mg/L,with a determination coefficient of R2=0.999 6.Under the addition levels of 2 500.00,625.00,and 156.25 mg/L,the average recovery rate of immunomodulatory octapeptin was 97.72%～101.32%,and the relative standard deviation(RSD)was 1.33%～3.94%.The stability results indicated that after 10 days of storage at 60℃,the appearance of the sample changed from light yellow powder to brownish-yellow powder,and both moisture content and immunomodulatory octapeptin content significantly decreased(P<0.05).After 10 days of storage under conditions of(25±2)℃and relative humidity of(90±5)%,the appearance of the sample changed from a light yellow powder to light yellow block,moisture content significantly increased(P<0.05),and immunomodulatory octapeptin content significantly decreased(P<0.05).After being packaged with aluminum foil+polyethylene(PE)composite material,and stored for six months under conditions of(40±2)℃and relative humidity of(75±5)%,the appearance of the sample showed no significant change,and neither moisture content nor immunomodulatory octapeptin content exhibited significant changes(P>0.05).The study indicates that detection method constructed in the experiment is accurate and stable,and is suitable for the quantitative analysis of immunomodulatory octapeptin.Immunomodulatory octapeptin is prone to changes in appearance under high-temperature and high-humidity conditions,and packaging with aluminum foil+PE composite material can effectively enhance its stability.关键词
免疫调节八肽/高温/高湿/稳定性Key words
immunomodulatory octapeptin/high-temperature/high-humidity/stability分类
农业科技引用本文复制引用
黄升,岳亮光,黄浩成,苏国旗,王力,杨飞云,黄金秀..免疫调节八肽检测方法的构建及其稳定性初步研究[J].饲料研究,2025,48(8):139-144,6.基金项目
国家生猪技术创新中心先导科技项目(项目编号:NCTIP-XD/B05) （项目编号:NCTIP-XD/B05）
国家重点研发计划课题(项目编号:2023YFD1301102) （项目编号:2023YFD1301102）
