生命科学研究2025，Vol.29Issue(2)：131-138,165,9.DOI:10.16605/j.cnki.1007-7847.2024.08.0168
幽门螺杆菌感染胃黏膜上皮细胞来源的外泌体HSPD1蛋白调控巨噬细胞分化
Exosomal HSPD1 Protein Derived from Helicobacter pylori Infected Gastric Mucosal Epithelial Cells Regulates Macrophage Differentiation
摘要
Abstract
In order to reveal the effect of exosomes derived from gastric mucosal epithelial cells infected with Helicobacter pylori on macrophage polarization,analyze the exosomal protein components and functions,exo-somes were collected from the supernatants of GES-1 gastric mucosal epithelial cells with and without H.pylori infection.Western-blot and flow cytometry were used to observe the effect of exosomes on macrophage polarization.Proteomic methods were employed to analyze and identify the differentially expressed proteins in exosomes derived from H.pylori-infected and non-infected GES-1 gastric mucosal epithelial cells,and Western-blot was used to verify the expression of these differentially expressed proteins in normal GES-1 and gastric cancer cells(MKN-7,AGS).Finally,immunohistochemistry was used to analyze the protein levels of heat shock protein family D member 1(HSPD1)in two groups of clinical tissue specimens,along with the expression levels of CD 163,a marker for M2-type macrophages.The results showed that exosomes from H.pylori-infected GES-1 cells promoted the expression of M2-type macrophage surface markers and polariza-tion-related proteins,including CD163,CD206,MGL1/2,and Arg-1.A total of 50 differentially expressed proteins were screened.The expression levels of HSPD1 in H.pylori-infected GES-1 cells and exosomes were significantly higher than those in the non-infected groups.In addition,the expression level of HSPD1 in H.pylori-positive gastric cancer(GC)tissues was significantly higher than that in H.pylori-negative GC tissues.In H.pylori-positive GC tissues,the expression level of CD 163 increased significantly and showed a significant positive correlation with the expression level of HSPD1 protein(r=0.472,P=0.01).These findings indicate that exosomes derived from H.pylori-infected gastric mucosal epithelial cells promote the M2-type polarization of macrophages.Among them,HSPD1 carried by exosomes,as a key regulatory factor,plays an indispensable role in driving the phenotypic transformation of macrophages.关键词
幽门螺杆菌/外泌体/热休克蛋白家族D成员1(HSPD1)/胃癌/肿瘤相关巨噬细胞(TAM)Key words
Helicobacter pylori/exosome/heat shock protein family D member 1(HSPD1)/gastric cancer/tumor-associated macrophage(TAM)分类
生物学引用本文复制引用
王楠,谢怡萍,李玉洁,翁轶玮,王建军..幽门螺杆菌感染胃黏膜上皮细胞来源的外泌体HSPD1蛋白调控巨噬细胞分化[J].生命科学研究,2025,29(2):131-138,165,9.基金项目
国家自然科学基金青年科学基金项目(82002111) （82002111）
昆山市第一人民医院医疗卫生科技创新专项(KETDCX202409) （KETDCX202409）
江苏省检验医学重点实验室开放课题项目(JSKLM-Z-2024-009) （JSKLM-Z-2024-009）
苏州市科教强卫面上项目(MSXM2024061) （MSXM2024061）
