一种特定合生元对高尿酸血症小鼠的干预作用OA

Hyperuricemia(HUA)has now become the second most common metabolic disease in China,fol-lowing diabetes.The regulation of intestinal flora by probiotics and prebiotics presents a promising method for the prevention and treatment of HUA.This study aimed to investigate the intervention effects of Lacto-bacillus gasseri and marine fish oligopeptides in HUA mice,which may provide an experimental basis for the development of food formulae for special medical purposes.The healthy mice were daily fed for four weeks with a HUA-inducing diet which contained yeast extracts,adenine,and potassium oxonate.During the period,mice in intervention group were daily administrated with 5×108 CFU L.gasseri or the synbiotic composed of 5×108 CFU L.gasseri and 3.75 mg marine fish oligopeptides.The results suggested that mice in HUA modeling group had significantly elevated serum levels of uric acid,urea and creatinine,as well as the activity of hepatic xanthine oxidase(XOD).Meanwhile,the HUA mice exhibited a significant reduction in intestinal microbial diversity,with significantly decreased abundances of Bacteroidota and Actinobacteriota,and significantly increased abundance of Proteobacteria.The contents of metabolites related to the protein digestion and absorption,and biosynthesis of amino acids and nucleotides were altered in gut.While both the probiotic and the synbiotic could prevent the HUA-induced changes in intestinal flora composition and metabolome.Compared with L.gasseri alone,the synbiotic could more effectively reduce the blood uric acid level in HUA mice,and significantly increase the excretion of uric acid in stool.