时珍国医国药2025，Vol.36Issue(9)：1619-1626,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-0904
基于JAK/STAT信号通路探讨大黄灵仙方干预Th17/Treg细胞平衡缓解肝内胆管炎症的作用机制
To explore the mechanism of Dahuang Lingxian Formula(大黄灵仙方)in intervening the Th17/Treg cell balance and relieving intrahepatic bile duct inflammation through regulating the JAK/STAT signaling pathway
摘要
Abstract
Objective To explore the effect of Dahuang Lingxian Formula(大黄灵仙方,DHLXF)on the balance of Th17/Treg cell subsets in rats with intrahepatic bile duct inflammation via regulating the JAK/STAT signaling pathway.Methods 25 rats were randomly divided into the blank group,the model group,the Danning Tablet group,the DHLXF low-dose and high-dose groups,with 5 rats in each.Except for the blank group,the remaining groups were administered1.25 mg/kg lipopolysaccharide(LPS)to establish a rat model of intrahepatic bile duct inflammation.During modeling,the Danning tablet group(0.5 g/kg),and the DHLXF low-dose(2.4 g/kg)and high-dose(4.8 g/kg)groups were administered intragastrically,while the blank group and the model group were given an equal volume of normal saline.After 8 days,hematoxylin-eosin(HE)was used to observe the degree of pathological changes in the rat liver tissues.The microplate method and enzyme-linked immunosorbent assay(ELISA)were used to detect serum inflammatory factor levels(ALT,AST,TBil,DBil,TBA,JAK1,STAT1,Foxp3,IL-10,IL-17,TNF-α).Western blot(WB)and real-time fluorescence quantitative PCR(RT-qPCR)were used to detect the expression levels of JAK1,STAT1,Foxp3,RORγt protein and mRNA in the bile duct tissues.Flow cytometry was used to detect peripheral blood helper T cell 17(Th17)and regulatory T cell(Treg)levels,and calcu-late the Th17/Treg ratio.One-way analysis of variance was used for comparison of measurement data among multiple groups,and LSD-t test was used for further pairwise comparison.Results Compared with the model group,the bile duct structure of the rats in Danning tablet group and the DHLXF low-dose and high-dose groups was more regular and more complete.The depth of inflammatory infiltra-tion in the portal area was greatly improved,and cell necrosis and congestion were rarely seen.The overall pathological morphology re-covered well,and the expression of ALT,AST,TBil,DBil,TBA,JAK1,STAT1,IL-17,TNF-α in the serum and the protein and mRNA expression of JAK1,STAT1,RORγt were decreased,and the expression of Foxp3,IL-10 content and Foxp3 protein and mRNA expression increased,the number of Treg cells in the peripheral blood of rats increased,the number of Th17 cells decreased,and the Th17/Treg ratio decreased(P<0.05).Conclusion DHLXF inhibits Th17 cell proliferation and induces Treg cell differentiation by reg-ulating the JAK/STAT signaling pathway and the expression of related inflammatory factors,maintains the balance of Th17/Treg cell sub-populations,and reduces the inflammatory stress response of rat intrahepatic bile duct cells,and promotes the biliary tract to return to a non-inflammatory state,thereby preventing and treating intrahepatic bile duct stones.关键词
大黄灵仙方/肝内胆管炎症/Janus 激酶/信号转导子与转录激活子/辅助性T细胞17/调节性T细胞Key words
Dahuang Lingxian Formula(大黄灵仙方,DHLXF)/Intrahepatic bile duct inflammation/Janus kinase/signal trans-ducer and activator of transcription/Helper T cells 17/regulatory T cells分类
医药卫生引用本文复制引用
付军,刘萌,俞渊,庞浇安,张曼,陈伟棠,马天如,叶桂源,罗艳萍,韦慧怡..基于JAK/STAT信号通路探讨大黄灵仙方干预Th17/Treg细胞平衡缓解肝内胆管炎症的作用机制[J].时珍国医国药,2025,36(9):1619-1626,8.基金项目
国家自然科学基金(82360889) （82360889）
第三批"岐黄工程"高层次人才团队培育项目(202411) （202411）
广西中医药大研究生教育创新计划项目(YCSY2023035) （YCSY2023035）
广西一流学科建设开放课题项目(2019XK062 （）
2019XK068) （）
广西中医药大学第一附属医院院级科研项目(2021QN001) （2021QN001）
广西中医药大学校级科研项目青年基金项目(2023QN008) （2023QN008）
