时珍国医国药2025，Vol.36Issue(9)：1627-1632,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0905
基于Hippo信号通路探讨麦门冬汤对肺纤维化大鼠的影响
Exploring the impact of Maimendong Decoction(麦门冬汤)on pulmonary fibrosis in rats based on the Hippo signaling pathway
摘要
Abstract
Objective To investigate the effects of Maimendong(Radix Ophiopogonis)Decoction(麦门冬汤,MMDD)on bleomycin(BLM)-induced pulmonary fibrosis(PF)in rats based on the Hippo signaling pathway.Methods Rats were randomly divided into 6 groups.A PF model was established by intratracheal injection of BLM,and samples were collected at 14 and 28 days after modeling.He-matoxylin-eosin(HE)staining,Masson staining,immunohistochemistry(IHC)and RT-qPCR were used to detect the morphological changes in the lung tissues and the levels of MST1,TGF-β1,Smad3 and Yap.Results HE and Masson staining revealed varying de-grees of pathological changes in the lung tissues across all groups compared to the blank group.After drug intervention,the morphological structure and fibrosis severity in the treatment groups were alleviated to varying degrees compared to the model group.IHC and PCR re-sults showed that,compared to the blank group,the protein and mRNA expression of MST1 were decreased at both time points,while the protein and mRNA expressions of TGF-β1,Smad3,and Yap were increased in varying degrees(P<0.05).Compared to the model group,the treatment groups exhibited increased MST1 expression and decreased protein and mRNA expressions of TGF-β1,Smad3,and Yap(P<0.05).Conclusion MMDD can ameliorate the progression of PF by regulating the Hippo signaling pathway.关键词
Hippo信号通路/麦门冬汤/肺纤维化/博来霉素/炎症因子Key words
Hippo signaling pathway/Maimendong Decoction(麦门冬汤,MMDD)/Pulmonary fibrosis(PF)/Bleomycin(BLM)/Inflammatory factors分类
医药卫生引用本文复制引用
龙婷,颜叶叶,赖春冰,吴一师,刘锐..基于Hippo信号通路探讨麦门冬汤对肺纤维化大鼠的影响[J].时珍国医国药,2025,36(9):1627-1632,6.基金项目
国家自然科学基金(82360911) （82360911）
第五批全国中医临床优秀人培养项目(国中医药人教函[2022]1号) （国中医药人教函[2022]1号）
广西青年岐黄学者培养项目(桂中医药科教发[2022]13号) （桂中医药科教发[2022]13号）
广西中医药慢性肺病防治重点研究室建设项目(桂中医药科教发[2023]9号) （桂中医药科教发[2023]9号）
