天津中医药大学学报2025，Vol.44Issue(5)：404-409,6.DOI:10.11656/j.issn.1673-9043.2025.05.04
超高效液相色谱指纹图谱及多成分定量对木贼的质量评价研究
Study on quality evaluation of Equiseti Hiemalis Herba by UHPLC fingerprint combined with multi-content determination
摘要
Abstract
[Objective]To establish ultra-high-performance liquid chromatography(UHPLC)fingerprint of Equiseti Hiemalis Herba and to comprehensively evaluate the quality of Equiseti Hiemalis Herba by combining chemometrics and multi-component content determination.[Methods]An ACQUITY CSH C18 chromatographic column was employed in this study,gradient elution was performed using 0.1％formic acid in water-acetonitrile as the mobile phase,and the detection wavelength was 270 nm.UHPLC fingerprints of 20 batches of Equiseti Hiemalis Herba were established,and the contents of components were determined.The fingerprints were evaluated by similarity analysis and hierarchical cluster analysis,and the differential components were found by orthogonal partial least squares discriminant analysis.[Results]There were 14 common peaks in the fingerprints of 20 batches of Equiseti Hiemalis Herba,and the similarity was greater than 0.992.Vanillic acid,kaempferol-3-O-sophoroside-7-O-glucoside,chlorogenic acid,ferulic acid,and kaempferol-3-O-sophoroside were identified by comparison with the standards.Hierarchical cluster analysis classified the 20 batches of Equiseti Hiemalis Herba into two categories,and orthogonal partial least squares discriminant analysis screened out six differential markers,and three components(kaempferol-3-O-sophoroside-7-O-glucoside,ferulic acid,vanillic acid)were identified.[Conclusion]The established analytical method is stable and reliable,and it can provide a reference for the quality evaluation of the Equiseti Hiemalis Herba.关键词
木贼/超高效液相色谱/指纹图谱/含量测定Key words
Equiseti Hiemalis Herba/UHPLC/fingerprint/content determination分类
中医学引用本文复制引用
武恬恬,赵鹏,吕振国,欧阳慧子,何俊..超高效液相色谱指纹图谱及多成分定量对木贼的质量评价研究[J].天津中医药大学学报,2025,44(5):404-409,6.基金项目
国家自然科学基金(82274091). （82274091）
