天津中医药大学学报2025，Vol.44Issue(5)：426-434,9.DOI:10.11656/j.issn.1673-9043.2025.05.07
火针疗法对大脑中动脉栓塞大鼠神经功能及肠道菌群的影响
Effects of fire-acupuncture therapy on neurological function and intestinal flora in MCAO rats
摘要
Abstract
[Objective]To observe the effect of fire needle on the neurological function and intestinal flora of rats with middle cerebral artery occlusion(MCAO)model,and to explore the mechanism of action of fire needle in the treatment of ischemic stroke.[Methods]SPF-grade healthy SD rats were divided into the sham operation group,MCAO group and fire-acupuncture group,in which the middle cerebral artery occlusion model was established in the MCAO group and fire-acupuncture group,and the sham operation group only carried out the separation of the common carotid artery,the internal carotid artery and the external carotid artery,and did not carry out the intervention of modelling;the fire-acupuncture group was subjected to the intervention of fire-acupuncture on the 2nd day after the completion of modelling,and the single-head fine fire-acupuncture of He was applied to the Zhongwan,Guan Yuan,Dazhui,and Mingmen.Rapid puncture was applied without needle retention,and the intervention was carried out once every other day,with a total of 4 interventions in 7 days.At the end of the intervention on the 7th day,Zausinger's six-point method was used to assess the neurological recovery of the rats,ELISA was used to detect the expression of IL-17 and TNF-α in the intestinal mucosa of the rats,HE staining was used to detect the overall structure of the intestinal mucosa of the rats,and the distribution of intestinal microorganisms was detected by 16SrRNA sequencing technology;ELISA was used to detect TNF-α in the peripheral blood and brain tissues of infarcted sides.TNF-α,IL-17 levels in brain tissue.[Results]Compared with the sham-operated group,the model group showed lower Zausinger score,higher levels of IL-17 and TNF-α in the intestinal mucosa,abnormal overall structure of the intestinal mucosa,lower abundance of intestinal microorganisms,more pathogenic bacteria,and higher levels of TNF-α and IL-17 in the peripheral blood and infarcted-side brain tissues(all P＜0.05),and the fire-acupuncture therapy significantly increased the MCAO rats' Zausinger score,decreased IL-17 and TNF-α levels in the intestinal mucosa,increased intestinal microbial diversity and decreased the relative abundance of pathogenic bacteria,and decreased TNF-α and IL-17 levels in peripheral blood and infarcted-side brain tissue(all P＜0.05).[Conclusion]Fire-needle therapy can promote the recovery of neurological function in MACO rats,and its mechanism may be related to the regulation of intestinal flora and the reduction of inflammatory response in peripheral and brain tissues.关键词
缺血性脑卒中/火针疗法/炎症因子/肠道菌群/大脑中动脉栓塞大鼠Key words
ischaemic stroke/fire needle therapy/inflammatory factors/intestinal flora/middle cerebral artery occlusion rat分类
中医学引用本文复制引用
曹璐璐,刘慧荣,李艳,王炳权..火针疗法对大脑中动脉栓塞大鼠神经功能及肠道菌群的影响[J].天津中医药大学学报,2025,44(5):426-434,9.基金项目
天津市名中医传承工作室建设项目 （）
上海市进一步加快中医药传承创新发展三年行动计划项目(2021-2023年)(ZY(2021-2023)-0205-01). （2021-2023年）
