土壤2025，Vol.57Issue(2)：300-307,8.DOI:10.13758/j.cnki.tr.2025.02.007
耐低温秸秆降解复合菌系的构建及功能验证
Construction and Functional Verification of Low-temperature-resistant Straw Degrading Synthetic Bacterial Consortium
摘要
Abstract
In order to improve the utilization of straw and increase soil organic matter by excavating low-temperature-resistant cellulose-degrading bacteria and constructing synthetic bacterial consortium(SynCom),carboxymethyl cellulose(CMC)selective medium and congo red discriminating medium were used to screen cellulose-utilizing strains from soil under long-term straw return.Through temperature control experiments,CMC enzyme activity and straw degradation rate were measured to explore the low-temperature degradation potential of the strains.Meanwhile,Arthrobacter sphaeroides AS-3 isolated from Antarctica(deposited in the Chinese Collection of Typical Cultures,CCTCC)was selected as a positive control,and the construction of low-temperature-resistant SynCom and the determination of the degradation level of maize stover were carried out.The results showed that the single bacteria CN8,CN10 and CN16(all are Bacillus sp.)effectively degraded corn straw at low temperature,while SynCom D constructed by the bacteria enhanced the degradation of corn straw more efficiently.The degradation rate of corn straw reached more than 26%after 30 d of incubation at a constant temperature of 15℃,which was more than the degradation rate of the single bacteria and the positive control strains.In conclusion,the low-temperature-resistant SynCom D has strong straw degradation ability,can enrich the beneficial microbial resources in the northeastern black soil,thus has a good application prospect and provide a theoretical basis for the utilization of straw resources in low-temperature habitats.关键词
秸秆降解/纤维素降解细菌/耐低温/复合菌系Key words
Straw degradation/Cellulose-degrading bacteria/Low temperature tolerance/Synthetic bacterial consortium分类
资源环境引用本文复制引用
赵丹,徐希媛,张玉潇,徐志辉,李琪,姚彦坡,褚海燕..耐低温秸秆降解复合菌系的构建及功能验证[J].土壤,2025,57(2):300-307,8.基金项目
国家重点研发计划项目(2022YFD1500202)和中国科学院战略性先导科技专项(A类)(XDA28020202)资助. （2022YFD1500202）
