土壤2025，Vol.57Issue(2)：342-352,11.DOI:10.13758/j.cnki.tr.2025.02.012
典型土壤活性组分对烟嘧磺隆微生物降解过程的影响与调节机制
Influence and Regulatory Mechanism of Typical Soil Active Components on Microbial Degradation of Nicosulfuron
摘要
Abstract
This study investigated the impact and regulatory mechanisms of soil active components—montmorillonite(MMT),humic acid(HA),and their composite(MMT-HA)on the microbial degradation of nicosulfuron using the highly efficient degradation fungus Talaromyces flavus LZM1.Advanced analytical techniques,including scanning electron microscopy coupled with X-ray energy-dispersive spectroscopy(SEM-EDS)and liquid chromatography-quadrupole time-of-flight mass spectrometry(LC-QTOF),were employed to systematically characterize the degradation process.The results demonstrated that individual or composite soil components(MMT,HA,or MMT-HA)enhanced microbial degradation activity by providing microbial colonization surfaces or energy metabolism substrates,which altered fungal hyphal morphology and shifted nicosulfuron degradation kinetics from first-order to zero-order.This not only enhanced microbial adaptability to high-pollution stress but also reduced the phase-transport potential and secondary toxicity of key degradation products such as 2-amino-4,6-dimethoxypyrimidine(ADMP).Furthermore,interactions between soil active components and microorganisms led to changes in nicosulfuron degradation pathways and product profiles.The results revealed that soil components stimulated microbial production of antioxidative metabolites(e.g.,orsellinic acid)to counteract oxidative stress,while the formation of orsellinic acid conjugates further reduced ADMP accumulation.Comparatively,MMT significantly inhibited hydroxylation product generation,whereas HA promoted alcoholysis product formation.These findings deepen the mechanistic understanding of nicosulfuron biodegradation and provide a scientific basis for optimizing microbial degradation technologies in agricultural soils contaminated with sulfonylurea herbicides.关键词
烟嘧磺隆/微生物降解/土壤活性组分/降解产物组成/调节机制Key words
Nicosulfuron/Microbial degradation/Soil active components/Degraded product composition/Regulatory mechanism分类
资源环境引用本文复制引用
高钲媛,谷成刚,范秀丽,沈乐祖,卞永荣,杨兴伦,王芳,蒋新..典型土壤活性组分对烟嘧磺隆微生物降解过程的影响与调节机制[J].土壤,2025,57(2):342-352,11.基金项目
中国科学院A类战略性先导科技专项课题(XDA28030501)、国家自然科学基金项目(42377406)和中国科学院南京土壤研究所自主部署项目(ISSAS2419)资助. （XDA28030501）
