土壤2025，Vol.57Issue(2)：477-484,8.DOI:10.13758/j.cnki.tr.2025.02.027
固氮蓝藻添加量对低温秸秆腐解和微生物碳源代谢活性的影响
Effects of Different Nitrogen Fixing Cyanobacteria Addition on Straw Decomposition and Microbial Carbon Metabolic Activities at Low Temperature
摘要
Abstract
A microcosm experiment was conducted using litter bag method to explore the changes of rice straw decomposition rate under different amounts of nitrogen-fixing cyanobacteria addition,in which four treatments were designed,i.e.,straw addition(CK),straw addition with low,medium and high amounts of cyanobacteria addition(LBA,MBA and HBA),and Biology method was used to determined carbon source metabolic activity potential(AWCD)and αdiversity of straw decomposing microorganisms.All treatments were incubated at 10℃for 90 days,straw samples were collected after 30,60,and 90 days to analyze straw decomposition rate,AWCD and αdiversity.The results showed that straw decomposition rate decreased with time under all treatments,but fast in the early stage and slow in the late stage.Moreover,AWCD,especially that related to labile carbon fractions,such as carbohydrates,decreased significantly.The amount of cyanobacteria addition affected straw decomposition rate,AWCD and αdiversity.Particularly,the highest straw decomposition rate was found under HBA within 30 days of decomposition,whereas which was occurred under LBA after 30 days of decomposition,meanwhile,the lowest straw decomposition rate appeared under MBA and HBA.The random forest analysis suggested that,during the 90 days of decomposition,the microbial degradation of recalcitrant C source,such as polymers,was the dominant factor responsible for the acceleration of straw decomposition under low amount of cyanobacteria addition,which may relate to the"microbial nitrogen mining"mechanism under low nitrogen condition.This study provides a fundamental understanding and a technical support on straw decomposition in paddy soils under cold-temperate climate.关键词
水稻秸秆/固氮蓝藻/秸秆腐解/微生物碳源代谢活性/微生物功能多样性Key words
Rice straw/Nitrogen-fixing cyanobacteria/Straw decomposition/Microbial carbon metabolic activity/Microbial functional diversity分类
农业科技引用本文复制引用
吴帆,樊聪聪,赵丽霞,梁玉婷,赵远,王晓玥..固氮蓝藻添加量对低温秸秆腐解和微生物碳源代谢活性的影响[J].土壤,2025,57(2):477-484,8.基金项目
中国科学院战略性先导科技专项(A类)攻关子课题(XDA28030102)和江苏省研究生科研与实践创新计划项目(SJCX24_1647)资助. （A类）
