土壤与作物2025，Vol.14Issue(2)：207-216,10.DOI:10.11689/sc.2024080701
大豆矮化突变体df1的候选基因鉴定
Candidate gene identification for soybean dwarf mutant df1
摘要
Abstract
Genetic mutations can be induced by external physical and chemical factors.This chromosomal genomic change not only creates new genetic variation materials for genetic improvement and molecular breeding of plants,but also serves as an important technical tool for studying gene function.In this study,a dwarf mutant,df1,was identified in the mutant library generated by gamma radiation of the widely cultivated cultivar Heihe 43 in Northeast China.Genetic analysis indicated that the df1 mutation is controlled by a single recessive gene.By analyzing the resequencing data of df1 and wild-type bulked pools sampled from a segregating line using the BVF-IGV pipeline,a large fragment deletion containing 69 genes on chromosome 1 is identified as the causal genetic factor for the df1 mutation.Association analysis using molecular markers derived from the large fragment deletion and transcrip-tome analysis of RNA-seq data of bulked df1 and wild-type supports the above conclusion.Twenty-six candidate genes including Glyma.01G066600 are finally determined by combined analysis of gene expression profiles and over 5 000 cultivars from the public database.The results of this study could facilitate the cloning of the functional gene for df1 mutation through fine-mapping and understanding of its molecular mechanism in regulating plant growth and development.关键词
大豆/矮化突变/大片段缺失/BVF-IGA/混池测序Key words
soybean/dwarf/large-fragment deletion/BVF-IGV/bulk sequencing分类
农业科学引用本文复制引用
刘锦宇,吴士浩,仲威宇,张文韬,朱晓斌,夏正俊..大豆矮化突变体df1的候选基因鉴定[J].土壤与作物,2025,14(2):207-216,10.基金项目
黑土地保护与利用科技创新工程专项资助(XDA28070400) （XDA28070400）
国家重点研发计划政府间国际科技创新合作(2022YFE0130300) （2022YFE0130300）
国家自然科学基金(U21A20215,32272094). （U21A20215,32272094）
