现代医药卫生2025，Vol.41Issue(5)：1087-1089,1094,4.DOI:10.3969/j.issn.1009-5519.2025.05.007
基层医院应用MPT64抗原鉴定非结核分枝杆菌可行性分析
Feasibility analysis of using MPT64 antigen to identify non-tuberculosis Mycobacteria in primary hospitals
摘要
Abstract
Objective To analyze the feasibility of MPT64 antigen identification of non-tuberculous My-cobacterium(NTM)in order to promote its application in primary hospitals.Methods A total of 1 177 sus-pected tuberculosis patients were cultured and isolated by MGIT960 automatic Mycobacterium rapid culture system in our hospital from January to December 2020.Targeted sequencing method was used as the gold standard for strain identification.Analyzed the ability of qPCR and MPT64 antigen colloidal gold method to i-dentify NTM.Results A total of 316 positive strains of Mycobacterium were isolated from 1 177 patients,with a culture positive rate of 26.85％.284 cases of Mycobacterium tuberculosis(MTB)and 32 cases of NTM were identified using the tNGS method,with a detection rate of 10.13％(32/316)for NTM.The sensitivity and specificity of qPCR for detecting MTB and NTM are 100.00％.The sensitivity of MPT64 antigen colloidal gold method for detecting MTB was 98.59％,specificity was 100.00％,and accuracy was 98.70％.Conclusion The MPT64 antigen colloidal gold method has high sensitivity and specificity for NTM identification.This method is simple to operate,does not require specific instruments,and is very suitable for primary hospitals.关键词
结核分枝杆菌/非结核分枝杆菌/MPT64抗原/基层医院Key words
Mycobacterium tuberculosis/Non-tuberculosis Mycobacterium/MPT64 antigen/Pri-mary hospitals分类
基础医学引用本文复制引用
田海兵,蒋慧,张晓波,徐佳敏,钟辉..基层医院应用MPT64抗原鉴定非结核分枝杆菌可行性分析[J].现代医药卫生,2025,41(5):1087-1089,1094,4.基金项目
上海市奉贤区科委社会类科技发展基金项目(奉科20221436). （奉科20221436）
