畜牧兽医杂志2025，Vol.44Issue(3)：40-43,4.DOI:10.7606/j.issn.1004-6704.2025.03.007
不同季节对西门塔尔牛活体采卵和体外胚胎生产的影响
Influence of Different Seasons on in Vivo Oocyte Retrieval and in Vitro Embryo Production in Simmental Cattle
摘要
Abstract
In order to further study the effect of in vitro embryo production and transplantation in Simmental cattle,and to effectively improve the efficiency of excellent core group of cows,and to quickly solve the problems of small number of excellent core breeding groups and insufficient supply of high-quality breeding sources.In provincial Simmental beef cattle breeding farm in Wuwei city,15 excellent core group donors aged 1-2 years were selected,and their oocytes extracted by ultrasound-guided follicle puncture(OPU)in different seasons.The oocytes were then subjected to in vitro fertilization(IVF),in vitro cul-ture,and cryopreservation.The data at each stage were ana-lyzed.The results showed that after optimization,the OPU-IVF process could collect average of 17.58 oocytes per do-nor,with a cleavage rate of 69.67％after in vitro fertiliza-tion,and aocyst rate of 36.19％.Each donor could produce an average of 3.75 OPU-IVF embryos,and the pregnancy rate after embryo transplantation was 53.37％.This study concluded that the success rate of in vitro embryo production and transplantation in Simmental cattle is affected by vari-ous,such as the age of the donor cows,nutritional status,hormone treatment plan,laboratory environmental condi-tions,in vitro culture,cryopreservation,and transplantation environment In Northwest China,the collection of oocytes from live Simmental cattle should be carried out in spring and autumn,with significantly better results than in winter(P＜0.5),which may be related to the climate conditions and the physiological cycle of the beef cattle.关键词
肉牛/活体采卵/体外胚胎/试验/分析Key words
beef cattle/ovum pick-up/in vitro embryos/experiment/analysis分类
畜牧业引用本文复制引用
张元来,杨成文,张杰,王红兵,阚丰年,孔伟..不同季节对西门塔尔牛活体采卵和体外胚胎生产的影响[J].畜牧兽医杂志,2025,44(3):40-43,4.基金项目
甘肃省农业农村厅科技支撑项目(KJZC-2024-10) （KJZC-2024-10）
东西部科技协作专项(24CXNH008) （24CXNH008）
