畜牧与兽医2025，Vol.57Issue(6)：6-12,7.
西藏娘亚牦牛细管冻精制作与人工授精试验
Production of frozen semen and test of artificial insemination in Xizang Niangya yak
摘要
Abstract
In order to explore the efficient propagation technique of Niangya yak,the semen of 3 Nyonya male yak was collected using the false vagina method,and the quality of the sperms was detected,and thin tube frozen semen was prepared.Then,fifty-six non-pregnant healthy female yak aged about 3 years old were selected for simultaneous estrus treatment with three drug combinations PG+PG+PMSG(Group A,n=16),Cue-Mate+PG+PMSG(Group B,n=20)and GnRH+PG+GnRH(Group C,n=20),and artificial insemination was performed.Finally,pregnancy detection was carried out with early pregnancy test card and portable B-ultrasound diagnostic instrument.The results showed that the average semen volume,sperm density,viability and deformability in Niangya yak were(2.03±0.25)mL,(14.04±2.52)×108/mL,(83.00±3.46)％and(5.33±1.53)％,respectively;which met the preparation criteria of frozen semen.After being thawed,the average sperm motility rate was(46.08±2.14)％,with a malformation rate of(10.72±5.81)％,a plasma membrane integrity rate of(47.69±1.42)％,and an acrosome integrity rate of(55.94±1.75)％.The results also showed that the estrus rate of Group B was 60％at 48 h,that of Group A was 37.5％at 48 h,that of Group C was the slowest,and that of Croup C was only 20％at 48 h and 60％at 96 h.The highest total estrus rate was 90％in Group B,which was not significantly different from 87.5％in Group A(P＞0.05),but signif-icantly different from 60％in Group C(P＜0.05).The pregnancy rates of the three groups were 28.57％,33.33％and 33.33％,respective-ly,with no significant difference(P＞0.05).This paper provided practical guidance for the preparation and artificial insemination of fine tube frozen essence in Niangya yak.关键词
娘亚牦牛/冻精制作/同期发情/人工授精/妊娠检测Key words
Niangya yak/frozen semen production/synchronous estrus/artificial insemination/pregnancy testing分类
畜牧业引用本文复制引用
昝瑞隆,符汉宇,欧杰次仁,平措卓嘎,次仁拉姆,李广玉,刘锁珠,梁春年,徐业芬..西藏娘亚牦牛细管冻精制作与人工授精试验[J].畜牧与兽医,2025,57(6):6-12,7.基金项目
西藏自治区科技厅区域科技协同创新专项(QYXTZX-NQ2022-04) （QYXTZX-NQ2022-04）
西藏自治区科技厅重点项目(XZ202001ZY0044N) （XZ202001ZY0044N）
西藏农牧学院大学生创新创业训练计划项目(2023-12) （2023-12）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
