畜牧与兽医2025，Vol.57Issue(6)：13-19,7.
猪体外胚胎第一次卵裂前后3'UTR组的动态变化
Dynamic change of 3'UTRome of porcine in vitro embryos during the first cleavage
刘小英 1王昕昕 1赵晗 1杜志强 1杨彩侠1
作者信息
- 1. 长江大学动物科学技术学院,湖北荆州 434025
- 折叠
摘要
Abstract
The study aims to analyze the dynamic changes of mRNA 3'UTRome during the first cleavage of porcine embryos derived from in vitro fertilization(IVF)or parthenogenetical activation(PA),providing useful reference for experimentally revealing the molecular mecha-nism of maternal mRNAs decay via 3'UTR at post-transcriptional level.Single-cell RNA-seq(scRNA-seq)data sets of porcine IVF and PA embryos at 1-and 2-cell stages were obtained from our previously published paper.DaPars software was used to identify the mRNAs with 3'UTR difference,and the signal pathways were enriched from these identified mRNAs.The mRNA 3'UTRs with great differences were se-lected to respectively visualize the length of 3'UTR region.The cis-functional elements cytoplasmic poly(A)element(CPE),poly(A)signal(PAS),microRNA binding sites and m6A sites contained in 3'UTRs with significant length difference were predicted using different softwares.The results showed that 207 mRNAs in the porcine IVF embryos and 117 mRNAs in the porcine PA embryos were identified with significantly different 3'UTRs(P＜0.05)during the first cleavage.These mRNAs were involved in different GO and KEGG pathways,respec-tively.Further analysis showed the difference of mean 3'UTR length of KPNA3 in the IVF group and HMGB1,CSNK1G3 and RASSF3 in the PA group to be greater than 90 nt.There were differences in the location,number or types of CPE and PAS contained within 3'UTRs of these 4 mRNAs.Of the microRNA binding sites contained within 3'UTRs of KPNA3 and CSNK1G3,and m6A sites were contained within 3'UTRs of CSNK1G3.These results indicated that the dynamic changes of 3'UTRs existed in many mRNAs of porcine IVF and PA embryos during the first cleavage.3'UTRs of KPNA3 in the IVF group and HMGB1,CSNK1G3 and RASSF3 in the PA group were of great length difference.The abundance of these mRNAs were possibly regulated by the different functional elements contained within their 3'UTRs.关键词
猪/体外受精胚胎/孤雌激活胚胎/DaPars/3'UTR组Key words
pig/IVF embryo/PA embryo/DaPars/3'UTRome分类
畜牧业引用本文复制引用
刘小英,王昕昕,赵晗,杜志强,杨彩侠..猪体外胚胎第一次卵裂前后3'UTR组的动态变化[J].畜牧与兽医,2025,57(6):13-19,7.
